F-12 K medium (BOSTER, USA), fetal bovine serum (Life Technology 10,099,141, USA), horse serum, DMSO (Solebao, China), penicillin/streptomycin (Yuanpei, China), trypan blue dye Liquid (Melen, China), trypsin-EDTA, BCA protein content determination kit (Jin Yibai, China), poly-L-lysine (source leaf, China), nerve growth factor (Boaosen, China), NSE, MAP 2 antibody (abcam, USA), Grp78, CHOP antibody (Santa Cruz, USA), secondary antibody (Bio-Rad, USA), thapsigargin (TG), icariin (crystal pure, China), CCK8 kit (Biosharp, China), Sulfa Rhodamine B (SRB) Kit (Beibo, China), Annexin V-FITC Apoptosis Kit (Biyuntian, China), TaKaRa PrimeScriptTM RT Master Mix Reverse Transcription Kit, TaKaRa TB GreenTM Premix Ex Taq TMII PCR kit (TaKaRa, Japan) .
F12k medium
Manufactured by Boster Bio
Sourced in United States
F12K medium is a cell culture medium formulated to support the growth of various cell lines, including human and animal cell lines. It provides a balanced combination of nutrients, amino acids, vitamins, and inorganic salts essential for cell proliferation and maintenance in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Tb greentm premix ex taqtmii pcr kit, by Takara Bio (1 mentions)
Primescripttm rt master mix reverse transcription kit, by Takara Bio (1 mentions)
Cck 8 kit, by Biosharp (1 mentions)
Streptomycin, by Boster Bio (1 mentions)
Penicillin, by Boster Bio (1 mentions)
Mccoy s 5a medium, by Sartorius (1 mentions)
Rpmi 1640 medium, by Sartorius (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Beyotime (1 mentions)
H2030, by Cobioer Biosciences (1 mentions)
H1975, by Cobioer Biosciences (1 mentions)
Hcc827, by Cobioer Biosciences (1 mentions)
Dmem f12 medium, by Corning (1 mentions)
A549 cell line, by American Type Culture Collection (1 mentions)
Imr 90 cell line, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions)
6 protocols using f12k medium
1
Neuroprotective Effects of Icariin in ER Stress
2
Culturing HUVECs for Atherosclerosis Modeling
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Human umbilical vein endothelial cells (HUVECs) were provided by The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences and maintained at 37˚C in a humidified incubator with 5% CO2. HUVECs were cultured in F12K medium (Boster Biological Technology Co., Ltd.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 µg/ml streptomycin and 100 IU/ml penicillin (Boster Biological Technology Co., Ltd.). HUVECs were treated with 100 µg/ml oxidized low-density lipoprotein (ox-LDL; UnionBiol Biotechnologies Co., Ltd.) for 24 h at 37˚C to simulate atherosclerosis, as previously described (20 (link)).
3
Cell Line Cultivation Protocol
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Human cell lines T24, EJ, 253J, and 5637 and the immortalized normal
uroepithelium cell line SV-HUC-1 came from our laboratory (Key Laboratory of
Urology, Second Hospital of Tianjin Medical University). Among them, T24 was
cultivated in McCoy’s 5A medium (BIOIND, Kibbutz Beit Haemek, Israel), EJ was
maintained in F12K medium (BOSTER, Wuhan, China), and 253J and 5637 were
fostered in RPMI 1640 medium (BIOIND). All media were complemented with 10%
fetal bovine serum (FBS) and 1% penicillin-streptomycin, and all cell lines were
cultured in a humidified incubator at 37°C with 5% CO2.
uroepithelium cell line SV-HUC-1 came from our laboratory (Key Laboratory of
Urology, Second Hospital of Tianjin Medical University). Among them, T24 was
cultivated in McCoy’s 5A medium (BIOIND, Kibbutz Beit Haemek, Israel), EJ was
maintained in F12K medium (BOSTER, Wuhan, China), and 253J and 5637 were
fostered in RPMI 1640 medium (BIOIND). All media were complemented with 10%
fetal bovine serum (FBS) and 1% penicillin-streptomycin, and all cell lines were
cultured in a humidified incubator at 37°C with 5% CO2.
4
Culturing Bladder Cancer Cell Lines
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Human bladder cancer cell lines (T24, UMUC3, RT112, 5637) were cultured in Roswell Park Memorial Institute medium‐1640 (RPMI‐1640; Gibco) supplemented with 10% foetal bovine serum (FBS) and 1% penicillin–streptomycin solution (100×; Beyotime). The SVHUC cell line was cultured in F12K medium (Boster) supplemented with 10% FBS and 1% penicillin–streptomycin solution. All cells were cultured at 37°C and 5% CO2 in a cell incubator.
5
Lung Adenocarcinoma Cell Line Characterization
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The human lung adenocarcinoma A549 (ATCC Cat# CRL-7900), HCC827 (KCLB Cat# 70,827), H1975 (ATCC Cat# CRL-5908), H2030 (ATCC Cat# CRL-5914), H23 (ATCC Cat #CRL-5800), and PC9 (BCRJ Cat #0331) cell lines were obtained from Cobioer Biosciences (Nanjing, China). Short tandem repeat (STR) analyses of the A549, HCC827, and H1975 cell lines were performed in 2017, and STR analyses of the H2030 and PC9 cell lines were performed in 2019. A549 cells were maintained in F12K medium (Boster, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Grand Island, NY, USA), 100 U/mL penicillin, and 0.1 mg/mL streptomycin (HyClone, Logan, UT, USA). The remaining cell lines were cultured in RPMI 1640 medium (HyClone, Omaha, NE, USA). The LLC cell line (ATCC Cat #CRL-1642) was maintained in DMEM (HyClone) supplemented with 10% FBS. All cell lines were incubated at 37 °C in a humidified incubator with 5% CO2. All cell lines were confirmed to be Mycoplasma-negative (Biothrive Sci. & Tech. Ltd., Shanghai, China), and the cell lines were used within 3 months after resuscitation.
6
TGF-β-Induced Epithelial-Mesenchymal Transition
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The A549 cell line and IMR-90 cell line were purchased from ATCC. A549 cells were cultured in F-12K medium (Boster Biological Technology) containing 10% foetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin. IMR-90 cells were cultured in DMEM (Biological Industries) containing 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. The MLE-12 cell line was purchased from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). MLE-12 cells were cultured in DMEM/F-12 medium (Corning) containing 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. A549, MLE-12, and IMR-90 cells were incubated in a humidified incubator with 95% air and 5% CO2 at 37 °C. A549 cells, MLE-12 cells, and IMR-90 cells were seeded in six-well plates and cultured overnight. Cells were treated with TGF-β (10 ng/mL) for 24 h[37 (link)] and then exposed to 680C91 (20 μm) for 24 h.
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