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Anti cd8β

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Anti-CD8β is a monoclonal antibody that binds to the CD8β subunit of the CD8 co-receptor expressed on the surface of cytotoxic T cells. It is used in flow cytometry applications to identify and quantify CD8+ T cell populations.

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Lab products found in correlation

Click it plus edu alexa fluor 647 flow cytometry assay kit, by Thermo Fisher Scientific (2 mentions) Anti cd8α, by Thermo Fisher Scientific (2 mentions) Anti vγ1 clone 2, by BioLegend (2 mentions) Anti cd3, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (2 mentions)

Close spelling variants of this product

Anti-CD8β-PE (2 citations) Rat anti-mouse CD8β eFlour 450 (2 citations)

2 protocols using anti cd8β

1

Isolation and Characterization of Intraepithelial Lymphocytes

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SI IELs were isolated as previously described7 (link) and GFP+ γδ IELs were sorted to 98% purity using a BD FACSAria II. IELs were stained with viability dye (eFluor 450 or eFluor 780), anti-CD3 (2C11), anti-CD8α (53–6.7), anti-CD8β (H35–17.2), anti-CD4 (GK1.5), anti-TCRβ (H57-597), anti-TCRγδ (GL3) (eBioscience), anti-Vγ7 (clone GL1.7; Rebecca O’Brien, National Jewish Health, Denver, CO), and anti-Vγ1 (clone 2.11, BioLegend) or a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen). Flow cytometry was performed on an LSR Fortessa (BD Biosciences) in the New Jersey Medical School Flow Cytometry and Immunology Core Laboratory. Data were analyzed by FlowJo (v.10.4.0; Tree Star).
Jia L., Wu G., Alonso S., Zhao C., Lemenze A., Lam Y.Y., Zhao L, & Edelblum K.L. (2022). A transmissible γδ intraepithelial lymphocyte hyperproliferative phenotype is associated with the intestinal microbiota and confers protection against acute infection. Mucosal immunology, 15(4), 772-782.
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2

Isolation and Characterization of Intraepithelial Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
SI IELs were isolated as previously described7 (link) and GFP+ γδ IELs were sorted to 98% purity using a BD FACSAria II. IELs were stained with viability dye (eFluor 450 or eFluor 780), anti-CD3 (2C11), anti-CD8α (53–6.7), anti-CD8β (H35–17.2), anti-CD4 (GK1.5), anti-TCRβ (H57-597), anti-TCRγδ (GL3) (eBioscience), anti-Vγ7 (clone GL1.7; Rebecca O’Brien, National Jewish Health, Denver, CO), and anti-Vγ1 (clone 2.11, BioLegend) or a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen). Flow cytometry was performed on an LSR Fortessa (BD Biosciences) in the New Jersey Medical School Flow Cytometry and Immunology Core Laboratory. Data were analyzed by FlowJo (v.10.4.0; Tree Star).
Jia L., Wu G., Alonso S., Zhao C., Lemenze A., Lam Y.Y., Zhao L, & Edelblum K.L. (2022). A transmissible γδ intraepithelial lymphocyte hyperproliferative phenotype is associated with the intestinal microbiota and confers protection against acute infection. Mucosal immunology, 15(4), 772-782.
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