Fry fish at 0 dph were fixed in 4% paraformaldehyde/PBS at 4 °C overnight, embedded in paraffin, and sectioned serially at 10 μm thickness. They were then stained with the Apoptotic/Necrotic Cell Detection kit (Takara Bio Inc.) and mounted with Vectashield HardSet mounting medium with DAPI H-1500 (Vector Laboratories Inc., Burlingame, CA). All germ cells and gonadal somatic cells were counted based on DAPI staining under a Fluoview FV10i confocal microscope (Olympus, Tokyo, Japan).
Dapi h 1500
Manufactured by Vector Laboratories
Sourced in United States
DAPI H-1500 is a fluorescent dye used for nucleic acid staining. It is a blue-fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA.
Automatically generated - may contain errors
Lab products found in correlation
Fluoview fv10i confocal microscope, by Olympus (1 mentions)
Vectashield hardset mounting medium, by Vector Laboratories (1 mentions)
Rna extraction kit, by Zymo Research (1 mentions)
Anti gapdh g9545, by Merck Group (1 mentions)
Anti n cadherin 610921, by BD (1 mentions)
Axioplan 2, by Zeiss (1 mentions)
Rabbit anti ki67, by Thermo Fisher Scientific (1 mentions)
Nunc lab tek 2 chamber slide system, by Thermo Fisher Scientific (1 mentions)
Thunder imaging system, by Leica (1 mentions)
4 protocols using dapi h 1500
1
Quantifying Apoptosis in Fish Germ Cells
2
Molecular Mechanisms of Cancer Metastasis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gold (III) chloride (520918,), Sodium Citrate (C8532), gemcitabine (G6423), and Thiazolyl Blue Tetrazolium Bromide (MTT) (M6494) were purchased from Sigma (St. Louis, MO, USA). Matrigel (CACB354234) and transwell (89235-020) were bought from VWR (Radnor, PA, USA). Alexa Fluor 488-conjugated secondary antibody (R37114) and Alexa Fluor 568-Phalloidin (A12380) were purchased from Life technology (Waltham, MA, USA). The RNA extraction kit is from ZYMO research (R1055, Irvine, CA, US). cDNA transcription kit (1708891) and the qPCR kit (1708882, iQ SYBR Green Supermix) were from Bio-Rad (Hercules, CA, USA).
Anti-E-cadherin (610182) and anti-N-cadherin (610921) were from BD Bioscience ( San Jose, CA, USA); anti-Vimentin (5741), anti-p38(8690), anti-phospho p38(9211), anti-p42/44 (9102), and anti-phospho p42/44(4370) were from Cell Signaling (Danvers, Massachusetts, US); anti-GAPDH(G9545, Sigma, St. Louis, MO, USA), anti-Tubulin(ab18207, Abcam, Cambridge, MA), DAPI( H-1500, Vector Laboratories, Burlingame, CA, USA).
Anti-E-cadherin (610182) and anti-N-cadherin (610921) were from BD Bioscience ( San Jose, CA, USA); anti-Vimentin (5741), anti-p38(8690), anti-phospho p38(9211), anti-p42/44 (9102), and anti-phospho p42/44(4370) were from Cell Signaling (Danvers, Massachusetts, US); anti-GAPDH(G9545, Sigma, St. Louis, MO, USA), anti-Tubulin(ab18207, Abcam, Cambridge, MA), DAPI( H-1500, Vector Laboratories, Burlingame, CA, USA).
3
Assess ChREBPβ Regulation of Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
INS-1–derived 832/13 cells grown in Nunc Lab-Tek II Chamber Slide System (Thermo Fisher, Cat. #154534) were transfected with ChREBPβ siRNA for 48 h. Cells were then cultured in 2 mmol/L or 20 mmol/L glucose for 16 h, and 100 μmol/L BrdU was added for 30 min. Cells were fixed with 2% paraformaldehyde, and treated with 1 N HCl for 30 min before treatment with 4% normal goat serum, 1% BSA, and 0.5% Triton for 2 h. Cells were incubated overnight with a BrdU antibody (Abcam, Cat. #ab6326) at 1:200. After washing with PBS twice, Alexa 594 anti-rat (1:10,000) was used as the second antibody. The slides were mounted with mounting medium containing DAPI (H-1500; Vector Laboratories, Inc.). To determine proliferation in dispersed rat islets, cells were stained for Ki67 using rabbit anti-Ki67 (Thermo Fisher Scientific, Cat. #9106). All images were acquired using a Zeiss Axioplan 2 at the Microscopy Shared Resource Facility of Icahn School of Medicine at Mount Sinai.
4
RNAscope Fluorescent In Situ Hybridization of Mfge8 mRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin-fixed and paraffin-embedded tissue sections (5-µm thickness) were used with the RNAscope Fluorescent Reagent Kit v2 and an RNAscope probe for mouse Mfge8 mRNA (Cat# 408771, Advanced Cell Diagnostics, Newark, CA, USA). A standard protocol used by our laboratory [29 (link)] for RNAscope fluorescent in situ hybridization was followed. After staining, slides were mounted with the antifade mounting media containing 4′, 6-diamidino-2-phenylindole (DAPI, H-1500, Vector Laboratories, Burlingame, CA, USA) and images were acquired using THUNDER Imaging Systems (Leica Microsystems, Wetzlar, Germany). Images were processed with Adobe Photoshop software (CC 2020).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!