Sciclone liquid handler

Manufactured by PerkinElmer

The Sciclone liquid handler is a laboratory instrument designed for automated liquid handling tasks. It is capable of precisely pipetting and processing small volumes of liquids with high accuracy and repeatability. The Sciclone provides a versatile platform for a range of applications in life science research and diagnostics, enabling efficient and consistent sample preparation and liquid handling workflows.

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Lab products found in correlation

Mosquito x1, by EQT (1 mentions) Pherastar, by BMG Labtech (1 mentions) Human gene 1.0 st array, by Thermo Fisher Scientific (1 mentions) Hiseq machine, by Illumina (1 mentions)

Spelling variants of this product

Sciclone G3 Liquid Handler (7 citations) Sciclone NGS Liquid Handler (5 citations)

2 protocols using sciclone liquid handler

Acinetobacter baumannii fluorescence assay

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A. baumannii 17978/p and 17978/pgfp cultures were grown O/N as above. Cells were washed as described above for the lipase assay. Compounds in DMSO or the DMSO control were added to the wells of a black low-volume Greiner 784073 plate using Perkin Elmer Sciclone liquid handler with a 50 nl pintool attachment calibrated to deliver 200 nM. For concentration-response assays, the TTP LabTech Mosquito X1 was used to place variable volumes (0.02–1.2 μl). Cultures were diluted to OD₆₀₀ = 0.005 in LB supplemented with 75 μM IPTG. Plates were centrifuged for 1 min at 1,000 rpm to ensure all liquid was at the bottom of the well and incubated overnight at room temperature in a humidified incubator. Fluorescence was measured after growth using a BMGLabtech PHERAstar (485 nm excitation, 520 nm emission). All data were uploaded to MScreen.

Waack U., Johnson T.L., Chedid K., Xi C., Simmons L.A., Mobley H.L, & Sandkvist M. (2017). Targeting the Type II Secretion System: Development, Optimization, and Validation of a High-Throughput Screen for the Identification of Small Molecule Inhibitors. Frontiers in Cellular and Infection Microbiology, 7, 380.

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Poly-A Selected RNA-Seq and Microarray

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Starting with total RNA, poly-A selected, non-stranded RNA-Seq libraries were constructed on the Sciclone liquid handler (Perkin Elmer). Forty bp single end reads were obtained using Illumina HiSeq machine (Illumina, San Diego, CA). For microarray analysis, total LP RNA was applied to Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA).

Dreijerink K.M., Groner A.C., Vos E.S., Font-Tello A., Gu L., Chi D., Reyes J., Cook J., Lim E., Lin C.Y., de Laat W., Rao P., Long H, & Brown M. (2017). Enhancer-mediated oncogenic function of the menin tumor suppressor in breast cancer. Cell reports, 18(10), 2359-2372.

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