Anti p p38 9211

BTD and DiaAlk were homemade; Catalase (C9322), Iodoacetamide (V900335), Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (678937), and sodium ascobate (A7631) were purchased from Sigma-Aldrich; dithiothreitol (A100281) was purchased from BBI Life Sciences; IPM (EVU111), Light Azido-UV-Biotin (EVU102), and Heavy Azido-UV-Biotin (EVU151) were purchased from KeraFast; CuSO₄ (C493-500) was purchased from Thermo Fisher Scientific; Sequencing grade trypsin (V5113) was purchased from Promega. Anti-actin antibody (A5441) (1:500) and anti-tubulin antibody (T9026) (1:500) were purchased from Sigma-Aldrich; Anti-p-eIF2α (3597) (1:1000) and anti-p-p38 (9211) (1:500) antibodies were purchased from Cell Signaling Technology; Anti-PMK-1 antibody (1:500) was a kind gift from Dr. R. Pukkila-Worley at University of Massachusetts Medical School, Worcester, MA^{58 (link)}; Anti-PRDX-2 antibody (1:2000) was a kind gift from Dr. E. Veal at Newcastle University Biosciences Institute, Newcastle upon Tyne, UK^{30 (link)}.

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and various other tissue culture reagents were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Primary antibodies are anti-iNOS, sc-650; anti-COX-2, sc-1745; anti-IκB-α, sc-371; anti-p-IκB-α, sc-8404; anti-p50, sc-7178; anti-p65, sc-8008, from Santa Cruz Biotechnology (Dallas, TX, USA) anti-p-ERK, #9101; anti-ERK, #9102; anti-p-JNK, #9251; anti-JNK, #9252S; anti-p-p38, #9211; anti-p38, #9212S, from Cell Signaling Technology (Danvers, MA, USA) Secondary antibodies: anti-mouse, ap124p; anti-goat, ap106p; anti-rabbit, ap132p, Millipore. The enzyme-linked immunosorbent assay (ELISA) kit for PGE₂ was purchased from R&D Systems (Minneapolis, MN, USA). The compounds glabridin, HSG4112, (S)-HSG4112, HGR4113 were provided by Glaceum Inc (Suwon, Republic of Korea).