Human saa elisa kit

Manufactured by Abcam

Sourced in United Kingdom, United States

The Human SAA ELISA kit is a quantitative in-vitro diagnostic test for the measurement of human serum amyloid A (SAA) levels in human serum or plasma samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique.

Automatically generated - may contain errors

Lab products found in correlation

Human mmp 9 quantikine elisa kit, by R&D Systems (1 mentions) Recombinant human il 1β, by Thermo Fisher Scientific (1 mentions) Human il 1 beta il 1f2 quantikine elisa kit, by R&D Systems (1 mentions) Spectramax m5, by Molecular Devices (1 mentions)

3 protocols using human saa elisa kit

SAA1 Regulation by IL-1β in CRC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

CRC cells were treated for 48 hours with or without recombinant human IL‐1β (10 ng/mL, PeproTech), after which SAA1 levels in culture supernatants were determined using a Human SAA ELISA kit (Abcam). Medium conditioned by macrophages was prepared as described above. Levels of IL‐1β and MMP‐9 in the CM were analyzed using a Human IL‐1 beta/IL‐1F2 Quantikine ELISA kit (R&D Systems) and a Human MMP‐9 Quantikine ELISA kit (R&D Systems) according to the manufacturer's instructions.

Sudo G., Aoki H., Yamamoto E., Takasawa A., Niinuma T., Yoshido A., Kitajima H., Yorozu A., Kubo T., Harada T., Ishiguro K., Kai M., Katanuma A., Yamano H., Osanai M., Nakase H, & Suzuki H. (2021). Activated macrophages promote invasion by early colorectal cancer via an interleukin 1β‐serum amyloid A1 axis. Cancer Science, 112(10), 4151-4165.

+ Open protocol

+ Expand

Serum SAA Level Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The serum contents of SAA were assessed by enzyme immunoassay procedure. SAA level was measured using a specific human SAA ELISA kit (Abcam Ltd., Cambridge, UK) according to the manufacturer’s instructions. The absorbance was determined at 450 nm.

Asadi H., Abolfathi A.A., Badalzadeh R., Majidinia M., Yaghoubi A., Asadi M, & Yousefi B. (2015). Effects of Ramadan Fasting on Serum Amyloid A and Protein Carbonyl Group Levels in Patients With Cardiovascular Diseases. Journal of Cardiovascular and Thoracic Research, 7(2), 55-59.

+ Open protocol

+ Expand

Quantification of Serum A-SAA in Liver Diseases

Check if the same lab product or an alternative is used in the 5 most similar protocols

The assessment of total A-SAA concentration in the sera from hepatitis, LC, and HCC patients was conducted using Human SAA ELISA kit (ab100635, Abcam, Cambridge, MA, USA). One hundred µL of 20 × PBS-diluted serum sample was added into the antibody-pre-coated ELISA plate and incubated at 4 °C overnight. Next, 100 µL of biotinylated SAA detection antibody was applied to the plate and recognized by addition of 100 µL of horseradish peroxidase-conjugated streptavidin. Chromogenic substrate was added and the absorbance at 450 nm was then measured using SpectraMax M5 microplate reader (Molecular Devices, San Jose, CA, USA).

Wu J.L., Su T.H., Chen P.J, & Chen Y.R. (2022). Acute-phase serum amyloid A for early detection of hepatocellular carcinoma in cirrhotic patients with low AFP level. Scientific Reports, 12, 5799.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!