Bacterial strains were analyzed by MALDI-TOF MS in an MBT Smart MALDI Biotyper (Bruker Daltonics, Bremen, Germany) using the updated database containing 9957 mass spectra profiles (MSPs). A few bacterial colonies of each enterococcal isolate were spotted onto the MALDI target plate. On-plate protein extraction was performed by applying 1 µL of formic acid on each target spot and letting it dry at room temperature before adding 1 µL of HCCA matrix solution (Bruker Daltonics, Bremen, Germany), following the manufacturer’s instructions. Spectra were acquired in positive mode in the range of 2000 to 20,000 Da, applying default settings [19 (link)].
Mbt smart maldi biotyper
Manufactured by Bruker
Sourced in Germany
The MBT Smart MALDI Biotyper is a compact, fully automated identification system for microorganisms. It uses matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry technology to rapidly identify and classify a wide range of microorganisms, including bacteria and yeasts, directly from sample material.
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Lab products found in correlation
4 protocols using mbt smart maldi biotyper
1
MALDI-TOF MS Identification of Enterococci
2
MALDI-TOF Analysis of Bacterial and Fungal Isolates
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MALDI-TOF MS was performed on 24-h-cultured isolates (Columbia blood agar [bioMérieux, France] for S. aureus and Candida Chrom-agar [Chrom-agar, United States] for C. albicans) based on a previous pilot study using 40 strains of S. aureus in which strains were equally classified using either 24-h-cultured or resuspended sessile cells grown in wells once biofilm had formed (data not shown). A colony of each strain was spotted on two positions of the MALDI-TOF MS plate and covered with 1 μL of 100% formic acid in order to lyse the cells and allow them to dry at room temperature. Then, 1 μL of organic matrix (α-cyano-hydroxycinnamic acid [HCCA, Bruker Daltonics, Bremen, Germany]) was added and allowed to dry. The plate was analyzed using an MBT Smart MALDI Biotyper (Bruker Daltonics) in the range between 2,000 and 20,000 Daltons.
3
Bacterial Identification by MALDI-TOF MS
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Bacterial strains were analyzed by MALDI-TOF MS in an MBT Smart MALDI Biotyper (Bruker Daltonics, Bremen) using the updated database containing 9957 Mass Spectra Profiles (MSPs). A few bacterial colonies of each enterococcal isolate were spotted onto the MALDI target plate. On-plate protein extraction was performed by applying 1µl formic acid on each target spot and let dry at room temperature before adding 1µl of HCCA matrix solution (Bruker Daltonics), following the manufacturer's instructions. Spectra were acquired in positive mode in the range of 2,000 to 20,000 Da, applying default settings (16) .
4
MALDI-TOF Biotyper Identification of Bacterial Isolates
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We identified the isolates using the MBT Smart MALDI Biotyper (Bruker Daltonics, Bremen, Germany). We spotted all strains from UHRC in duplicate onto the MALDI target plate and overlaid with 1 µl of 70% formic acid. After drying at room temperature, we covered and dried the spots with 1 µl HCCA matrix, according to the manufacturer's indications (Bruker Daltonics). We obtained two spectra in the range of 2,000-20,000 Da on each spot, resulting in 4 spectra per isolate. The isolates from UHB were analysed in one spot per strain and one spectrum from spot was acquired.
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