To determine the levels of TNF‐α, IL‐1β and MCP1, the supernatants were collected from the culures of NRK‐52E cells and subjected to ELISA analysis using specific kits. The kits used were TNF alpha Rat ELISA Kit (Invitrogen), IL‐1 beta Rat ELISA Kit (Invitrogen), and Rat MCP‐1 ELISA Kit (RayBiotech). The protocols were in accordance with the manufacturer‐provided manual.
Il 1 beta rat elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in Bulgaria
The IL-1 beta Rat ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of rat interleukin-1 beta (IL-1 beta) levels in cell culture supernatants, cell lysates, serum, and plasma samples.
Automatically generated - may contain errors
Lab products found in correlation
Tnf alpha rat elisa kit, by Thermo Fisher Scientific (3 mentions)
Scopolamine methyl nitrate, by Merck Group (2 mentions)
Rat mcp 1 elisa kit, by RayBiotech (1 mentions)
Phenazine methosulfate, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Nitroblue tetrazolium, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Pilocarpine, by Merck Group (1 mentions)
4 protocols using il 1 beta rat elisa kit
1
Cytokine Levels in NRK-52E Cells
2
Neurochemical Assays for Neuroprotection
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Phenazine methosulfate (PMS), nitroblue tetrazolium (NBT), trichloroacetic acid (TCA), thiobarbituric acid (TBA), 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), pilocarpine hydrochloride (Pilo), and scopolamine methyl nitrate were purchased from Sigma Aldrich (Hamburg, Germany). LCM (Vimpat, USB Pharma, Brussels, Belgium); TPM (Topamax, Janssen-Pharmaceutica NV, Beerse, Belgium); diazepam (Sopharma, Sofia, Bulgaria); IL-1 beta Rat ELISA Kit—Invitrogen (Thermo Fisher Scientific, Vienna, Austria). TNF alpha Rat ELISA Kit—Invitrogen, Thermo Fisher Scientific.
3
Quantifying IL-1β Secretion from NRK Cells
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Culture media were collected from NRK cells treated as indicated above. The collected media were centrifuged at 2000–3000 rpm for 20 min, and the supernatants were collected for detection of IL-1β secretion according to the manufacturer’s instructions of the IL-1 beta Rat ELISA Kit (Invitrogen).
4
Neuroinflammation and Oxidative Stress Assays
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Lacosamide (Vimpat, USB Pharma, Brussels, Belgium); pilocarpine (Sigma Aldrich, Hamburg, Germany); scopolamine methyl nitrate (Sigma Aldrich, Hamburg, Germany); diazepam (Sopharma, Sofia, Bulgaria); IL-1 beta Rat ELISA Kit-Invitrogen-Thermo Fisher Scientific and TNF alpha Rat ELISA Kit-Invitrogen-Thermo Fisher Scientific. Phenazine methosulfate (PMS), nitroblue tetrazolium (NBT), trichloroacetic acid (TCA), thiobarbituric acid (TBA), 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), paraformaldehyde, hematoxylin & eosin (FOT Ltd., Sofia, Bulgaria).
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