High sensitivity rna screen tape sample buffer

Manufactured by Agilent Technologies

The High Sensitivity RNA Screen Tape Sample Buffer is a solution designed to prepare RNA samples for analysis using the Agilent Bioanalyzer system. The buffer is formulated to optimize the performance of the RNA screen tape, ensuring accurate measurement of RNA concentration and integrity.

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Lab products found in correlation

Rneasy plus micro kit, by Qiagen (2 mentions) 2200 tapestation system, by Agilent Technologies (1 mentions) Mircury rna isolation kit cell and plant, by Qiagen (1 mentions) Turbo dna free kit, by Thermo Fisher Scientific (1 mentions) Qubit rna hs assay kit, by Thermo Fisher Scientific (1 mentions) Primepcr primers, by Bio-Rad (1 mentions) Ssoadvanced universal sybr green supermix, by Bio-Rad (1 mentions) Primepcr assay, by Bio-Rad (1 mentions) Iscript reverse transcription supermix, by Bio-Rad (1 mentions) Lightcycler 96, by Roche (1 mentions) Tetrad 2 peltier thermal cycler, by Bio-Rad (1 mentions)

Spelling variants of this product

RNA Screen Tapes (16 citations) RNA Screen Tape Sample Buffer (9 citations) High Sensitivity Screen Tape (4 citations)

2 protocols using high sensitivity rna screen tape sample buffer

RNA Extraction and Quantification Protocol

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For RNA subjected to the Clariom D microarray, the miRCURY RNA Isolation Kit – Cell and Plant (Exiqon) was used according to the manufacturer's instructions. Subsequently, DNase digest was performed using the Turbo DNA‐free kit (Invitrogen). Since the Exiqon kit was later discontinued, the RNeasy Plus Micro Kit (Qiagen) was used according to the manufacturer's instructions for all qRT‐PCRs and transfer experiments.
RNA concentration measurements were performed either with the Qubit RNA HS assay kit (ThermoFisher) or with the 2200 Tape Station System using the High Sensitivity RNA Screen Tape Sample Buffer (Agilent).

Budden C.F., Gearing L.J., Kaiser R., Standke L., Hertzog P.J, & Latz E. (2021). Inflammasome‐induced extracellular vesicles harbour distinct RNA signatures and alter bystander macrophage responses. Journal of Extracellular Vesicles, 10(10), e12127.

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Quantitative Real-Time PCR Validation Protocol

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Real time quantitative polymerase chain reaction (RT-qPCR) validation was done using PrimePCR™ Assays (Bio-Rad) with SYBR® chemistry. RNA was extracted using an RNeasy Plus Micro Kit (Qiagen, 74034), and checked for integrity using an Agilent 2200 Tapestation System with High Sensitivity RNA Screentape (Agilent, 5067-5579), High Sensitivity RNA ScreenTape Sample Buffer (Agilent, 5067-5580), and High Sensitivity RNA ScreenTape Ladder (Agilent, 5067-5581). cDNA was generated using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708840) with a Tetrad2 Peltier Thermal Cycler (Bio-Rad). PrimePCR™ Primers (Bio-Rad) used were as listed in Table 1. RT-qPCR was done with SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, 1725270) on a LightCycler96 (Roche). Technical replicates were carried out in triplicate. Analyses was done in Excel and GraphPad Prism Software. Ct values were normalized to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Outliers were detected using Grubbs' Test, with α=0.05. Results of RT-qPCR are presented as the average Relative Quantification (RQ=2 -ΔΔCt ) values and the upper and lower RQ of the biological replicates. Any undetected samples were given a Ct value of the maximum number of cycles plus 1.

Robinson M., Witherspoon L., Willerth S.M., & Flannigan R. (2021). Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells.

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