A specific volume of a dispersion of the MPs in the medium of desired concentration, was placed on a fibronectin-coated glass coverslip fitted in a magnetic imaging chamber (Chamlide CMB, CM-B25-1) filled with CO2-equilibrated culture medium. After a given time, ranging from few minutes for silica particles and after a few hours for polystyrene particles, aggregates were deposited at random on the coated glass coverslip. The final volume of cultured medium was 1.5 mL. The chamber was sealed with mineral oil to prevent water evaporation and placed for viewing in an inverted microscope (TIRF AF 6000LX, Leica) equipped with a ×10 0.30 NA objective. Videos were recorded with a CCD camera (Photometrics Cascade 512B, Roper Scientific) at an acquisition rate of 1 frame every 10 min. Images were exported from the instrument software in TIFF format and visualized using the ImageJ software package v.1.46r (National Institutes of Health, Bethesda, MD).
Tirf af 6000lx
Manufactured by Leica
The TIRF AF 6000LX is a high-performance widefield fluorescence microscope system designed for live-cell imaging. It offers Total Internal Reflection Fluorescence (TIRF) and Autofocus functionality to enable precise observation of dynamic processes at the cell-substrate interface.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tirf af 6000lx
1
Time-lapse Imaging of Particle Deposition
2
Time-lapse Imaging of Cellular Aggregates
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The polymer-coated coverslips were placed
on the bottom of a microscope magnetic observation chamber (Chamlide
CMB, CM-B25-1). Droplets containing cellular aggregates were removed
from the Petri dish lid with a pipet and placed on the polymer-coated
glass coverslips. The microscope cell was filled with CO2-equilibrated culture medium, consisting of DMEM supplemented with
10% (v/v) FBS and antibiotics (100 μg mL–1 streptomycin and 100 U mL–1 penicillin) to reach
a final volume of 1.5 mL. The chamber was sealed with mineral oil
to prevent water evaporation and placed for viewing in an inverted
microscope (TIRF AF 6000LX, Leica) equipped with a 10× 0.30 NA
objective. Videos were recorded with a CCD camera (Photometrics Cascade
512B, Roper Scientific) at an acquisition rate of 1 frame every 10
min, exported from the instrument software in TIFF format, and visualized
using the ImageJ software package v.1.46r (National Institutes of
Health, Bethesda, MD) at 37 °C.
on the bottom of a microscope magnetic observation chamber (Chamlide
CMB, CM-B25-1). Droplets containing cellular aggregates were removed
from the Petri dish lid with a pipet and placed on the polymer-coated
glass coverslips. The microscope cell was filled with CO2-equilibrated culture medium, consisting of DMEM supplemented with
10% (v/v) FBS and antibiotics (100 μg mL–1 streptomycin and 100 U mL–1 penicillin) to reach
a final volume of 1.5 mL. The chamber was sealed with mineral oil
to prevent water evaporation and placed for viewing in an inverted
microscope (TIRF AF 6000LX, Leica) equipped with a 10× 0.30 NA
objective. Videos were recorded with a CCD camera (Photometrics Cascade
512B, Roper Scientific) at an acquisition rate of 1 frame every 10
min, exported from the instrument software in TIFF format, and visualized
using the ImageJ software package v.1.46r (National Institutes of
Health, Bethesda, MD) at 37 °C.
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