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Pe rat anti mouse cd4

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The PE Rat Anti-Mouse CD4 is a fluorochrome-conjugated antibody that binds to the CD4 receptor on the surface of mouse T helper cells. This antibody is designed for flow cytometry applications to identify and quantify CD4-positive cells in mouse samples.

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Lab products found in correlation

Apc rat anti mouse cd8a, by BD (1 mentions) Pe conjugated anti cd11b, by BD (1 mentions) Apc cy7 conjugated anti cd45, by BD (1 mentions) Apc conjugated anti ly6g, by BD (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) 7 aminoactinomycin d, by BD (1 mentions) Zombie aqua live dead dye, by BioLegend (1 mentions) Fitc rat anti mouse cd45, by BioLegend (1 mentions) Facscanto b, by BD (1 mentions) Dnase 1 type 2, by Merck Group (1 mentions) Apc rat anti mouse cd3, by BioLegend (1 mentions) Collagenase 4, by Worthington (1 mentions)

2 protocols using pe rat anti mouse cd4

1

Multicolor Flow Cytometry Panel

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The cells were stained with allophycocyanin (APC)‐Cy7‐conjugated anti‐CD45 (BD Pharmingen), phycoerythrin (PE)‐conjugated anti‐CD11b (BD Pharmingen), APC‐conjugated anti‐Ly6G (BD Pharmingen), BV421‐conjugated anti‐lymphocyte antigen 6 complex locus C (Ly6C) (BD Horizon), FITC Rat Anti‐Mouse CD19 (Biolegend), BV421 Rat Anti‐Mouse CD3 (BD Pharmingen), PE Rat Anti‐Mouse CD4 (Biolegend) and APC Rat Anti‐Mouse CD8a (BD Pharmingen). Dead cells and debris were gated out using forward light scatter, side light scatter, and 7‐aminoactinomycin D (BD Biosciences, San Jose, CA). The fluorescence‐activated cell sorting analysis was performed on a FACS Canto II flow cytometer and analyzed using FlowJo software (BD Biosciences).
Yan H., Kawano T., Kanki H., Nishiyama K., Shimamura M., Mochizuki H, & Sasaki T. (2023). Role of Polymorphonuclear Myeloid‐Derived Suppressor Cells and Neutrophils in Ischemic Stroke. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(6), e028125.
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2

Tumor Immune Cell Profiling

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Tumors were harvested and digested with 20 ¼g/ml type II DNase I (Sigma, D4527) and 1 mg/mL collagenase IV (Worthington LS004186) to obtain single cell suspensions. Spleen and blood samples were processed with red blood cell lysis buffer (1 mM ammonium bicarbonate and 114 mM ammonium chloride). Antibodies (1:100 ratio) and Zombie Aqua™ live/dead dye (1:500 ratio) (Biolegend, 423102) were added to cell suspensions in PBS and incubated for 20 min on ice before flow analysis on BD FACS CantoB. Data were analyzed on FlowJo and represented as percentages of positive cells. Antibodies: FITC rat anti-mouse CD45 (Biolegend, 103108), APC rat anti-mouse CD3 (Biolegend, 100235), PE rat anti-mouse CD4 (Biolegend, 116005), and PE/Cyanine7 rat anti-mouse CD8a (Biolegend, 100721).
Xiao L., Somers K., Murray J., Pandher R., Karsa M., Ronca E., Bongers A., Terry R., Ehteda A., Gamble L.D., Issaeva N., Leonova K.I., O’Connor A., Mayoh C., Venkat P., Quek H., Brand J., Kusuma F.K., Pettitt J.A., Mosmann E., Kearns A., Eden G., Alfred S., Allan S., Zhai L., Kamili A., Gifford A.J., Carter D.R., Henderson M.J., Fletcher J.I., Marshall G., Johnstone R.W., Cesare A.J., Ziegler D.S., Gudkov A.V., Gurova K.V., Norris M.D, & Haber M. (2021). Dual targeting of chromatin stability by the curaxin CBL0137 and histone deacetylase inhibitor panobinostat shows significant preclinical efficacy in neuroblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(15), 4338-4352.
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