Formic acid, acetonitrile, saline, 17-β-estradiol, letrozole, calcein, hematoxylin and eosin, a von Kossa staining kit, and a tartrate-resistant acid phosphatase (TRAP) activity assessment kit were purchased from Sigma-Aldrich (St Louis, MO, USA). The toluidine blue staining solution was purchased from Leagene Inc. (Beijing, People’s Republic of China). The estradiol (E2) enzyme immunoassay (EIA) kit was purchased from BioCheck Inc. (Burlingame, CA, USA).
Von kossa staining kit
Manufactured by Merck Group
Sourced in United States
The Von Kossa staining kit is a laboratory tool used to detect the presence of calcium deposits in tissue samples. It provides a staining method that allows the visualization of calcium-containing structures under a microscope. The kit includes the necessary reagents and instructions for performing the staining procedure.
Automatically generated - may contain errors
Lab products found in correlation
17β estradiol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Hematoxylin and eosin, by Merck Group (1 mentions)
Calcein, by Merck Group (1 mentions)
Letrozole, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Df6437, by Affinity Biosciences (1 mentions)
Anti trim25 67314 1 ig, by Proteintech (1 mentions)
Ab138492, by Abcam (1 mentions)
Af5484, by Affinity Biosciences (1 mentions)
Af0639, by Affinity Biosciences (1 mentions)
Senescence associated β galactosidase sa β gal staining kit, by Beyotime (1 mentions)
3 protocols using von kossa staining kit
1
Estradiol and Letrozole Effects Assessment
2
Quantifying Aortic Root Calcification
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After the experiment, blood was collected from which serum was isolated. A 10-min systemic perfusion was performed from the left ventricle using ice-cold phosphate-buffered saline (PBS) containing 1 mM EDTA, after which the aortic root was collected. The frozen cross-sectional samples of the aortic root were stained with von kossa staining kit (Sigma). Briefly, von kossa staining including the procedure of staining the sections in silver nitrate solution for 25 min and then exposing to ultraviolet light for 5 min, subsequently turning the calcified areas into black. The images were analyzed with ImageJ. The specific approach incorporates the procedure of importing the image into ImageJ, performing a THRESHOLD operation on the vascular region, adjusting the threshold to just exclude non-specific sites, and applying the same threshold to all images. The size of the calcified lesions in aortic root was expressed in μm2.
3
Morusin Regulates Osteoblast Differentiation
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Chemicals used in the study were procured from different sources: morusin (S0915) from Selleck Chemicals, DAPI (D8417) and dimethyl sulfoxide (C6164) from Sigma Aldrich, Nrf2 inhibitor ML385 (S8790) from Proteintech, Von Kossa staining kit from Sigma–Aldrich, Oil Red O staining kit (G1015) from Sigma–Aldrich, and senescence‐associated β‐galactosidase (SA‐β‐gal) staining kit from Beyotime. Additionally, various assay kits such as triglyceride assay kit (A110‐1‐1), total cholesterol assay kit (A111‐1‐1), high‐density lipoprotein cholesterol assay kit (A112‐1‐1), and low‐density lipoprotein cholesterol assay kit (A113‐1‐1) were procured from Nanjing Jiancheng Bioengineering Institute. The antibodies used for immunoblot, immunofluorescence, and immunohistochemical staining assays were as follows: anti‐actin (81115‐1‐RR, Proteintech), anti‐P21 (2947S, CST), anti‐RUNX2 (8486, CST), anti‐ALP (MAB29092, R&D), anti‐TRIM25 (67314‐1‐Ig, Proteintech), anti‐TRIM16 (24403‐1‐AP, Proteintech), anti‐KEAP1 (10503‐2‐AP, Proteintech), anti‐NQO1 (DF6437, Affinity), anti‐HMXO‐1 (DF8020, Affinity), anti‐NRF2 (AF0639, Affinity), anti‐Cyclin D1 (DF6386, Affinity), anti‐P53 (AF0865, Affinity), anti‐P16 (AF5484, Affinity), anti‐IL6 (21865‐1‐AP, Proteintech), anti‐NF‐κB (66535‐1‐Ig, Proteintech), and anti‐collagen I (ab138492, Abcam).
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