OVA was purchased from Alfa Aesar Chemical Co., Ltd. (Karlsruhe, Germany). Dexamethasone (DXM), Al(OH)3, phorbol 12-myristate 13-acetate (PMA), and ionomycin were all obtained from Sigma-Aldrich Co., Ltd. (St. Louis., MO, USA). Allophycocyanin-conjugated anti-mouse CD4 antibody (APC-CD4), fluorescein isothiocyanate-conjugated anti-mouse IL-17 antibody (FITC-IL-17), phycoerythrin-conjugated anti-mouse CD25 antibody (PE-CD25), and the corresponding isotype controls were bought from eBioscience Inc. (San Diego, CA, USA). Brefeldin A was purchased from BD Pharmingen (San Diego, CA, USA). Enzyme-linked immuno sorbent assay (ELISA) kits for mouse interleukin- (IL-) 4, IL-6, IL-17a, IL-23, IL-25, tumor necrosis factor-α (TNF-α), and interferon gamma (IFN-γ) were bought from Bio-Swamp Life Science Lab (Wuhan, China). ELISA kits for total-lgE and lgG1 were obtained from BOSTER biological technology Co., Ltd. (Wuhan, China). Antibodies against AP-1 and actin beta (Actb) and HRP-conjugated goat anti-mouse secondary antibody were provided by Bioss Inc. (Beijing, China). Antibodies against phospho (p)-NF-κBp65 (Ser536), NF-κBp65, p-IκBα (Ser32), and IκBα were purchased from Cell Signaling Technology (Denver, MA, USA). All reagents for cell culture were acquired from Invitrogen Co., Ltd. (Paisley, UK). Other chemical reagents were of analytical grade.
Ifn γ
Manufactured by Bioswamp
Sourced in China
IFN-γ is a recombinant protein that functions as an interferon. Interferons are a group of signaling proteins involved in the immune response against viral infections and other biological activities.
Automatically generated - may contain errors
Lab products found in correlation
Primescript 2 rtase, by Takara Bio (2 mentions)
Bca protein concentration assay kit, by Solarbio (2 mentions)
Ionomycin, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions)
Brefeldin a, by BD (1 mentions)
Masson trichrome stain kit, by Solarbio (1 mentions)
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter gene assay kit, by Beyotime (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Annexin 5 pe 7 aad apoptosis assay kit, by BD (1 mentions)
Pentobarbital sodium, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Nisin staining solution, by Wuhan Servicebio Technology (1 mentions)
Dab concentrated kit, by Solarbio (1 mentions)
Hematoxylin, by Wuhan Servicebio Technology (1 mentions)
Neutral resin, by Sinopharm (1 mentions)
In situ cell death detection kit, by Solarbio (1 mentions)
Tnf α, by Bioswamp (1 mentions)
3 protocols using ifn γ
1
Murine Th17 Cell Regulation Protocol
2
Allergy-Induced Lung Inflammation Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following is a list of the main materials and reagents used in this study: OVA (A5253, sigma, Ronkonkoma, NY, USA); BCA Protein Concentration Assay Kit (PC0020, solarbio, Beijing, China); Oligo (dT)18/miR-RT Primer (3806, TAKARA, Kusatsu, Japan); PrimeScript II Rtase (2690A, TAKARA, Japan); T4 DNA ligase (Fermentas, Waltham, MA, USA); Dual luciferase reporter gene assay kit (RG027, Beyotime, Shanghai, China); Masson Trichrome Stain Kit (G1340, Solarbio, Beijing, China); ELISA kits IL-4 (HM10380), IL-5 (HM10213), IL-13 (HM10191) and IFN-γ (HM10058), purchased from Bioswamp(Wuhan, China); DAB Concentrate Kit (DA1010, Solarbio, Beijing, China); AnnexinV-PE/7 AAD Apoptosis Assay Kit (559763, BD, Becton Drive Franklin Lakes, NJ, USA).
3
Investigating 7,8-DHF Neuroprotective Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Para-chlorophenylalanine (PCPA) was purchased from Shanghai Genye. The 0.9% NaCl solution was adjusted to a weakly basic pH (pH 7.0–8.0) with NaOH and HCl to prepare a PCPA suspension (5% Tween-80) for use in this study. 7,8-Dihydroxyflavone (7,8-DHF) was purchased from MCE, pentobarbital sodium was purchased from Sigma Merck, dimethyl sulfoxide (DMSO) was purchased from Solepipe, and PEG300 was purchased from MCE. Eosin was purchased from Solarbio, hematoxylin was purchased from Servicebio, nisin staining solution was purchased from Servicebio, neutral resin was purchased from Shanghai Sinopharm, the In Situ Cell Death Detection Kit, the DAB concentrated kit was purchased from Solarbio, hematoxylin was purchased from Servicebio, the Marker was purchased from Helix, and the BCA protein concentration assay kit was purchased from Solarbio. ELISA kits for interleukin-1β (IL-1β), prostaglandins D2 (PGD2), melatonin (MT), corticosterone (CORT), IL-6, IL-10, tumor necrosis factor alpha (TNF-α), interferon-gamma (IFN-γ), and brain-derived neurotrophic factor (BDNF) were purchased from Bioswamp. TRIzol was purchased from Ambion, and PrimeScript II Rtase was purchased from TAKARA. Antibodies against Bcl-2, Bax, Bad, Caspase-3, TrKB, PI3K, Akt, cAMP, CREB, BDNF, GAPDH, p-TrKB, p-Akt and p-CREB, as well as goat anti-rabbit IgG, were purchased from CST.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!