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Esquire 6000 spectrometer

Manufactured by Bruker
Sourced in United States

The Esquire 6000 spectrometer is a laboratory instrument designed for mass spectrometry analysis. It is capable of performing high-resolution mass measurements and can be used for a variety of applications that require accurate mass determination.

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2 protocols using esquire 6000 spectrometer

1

Comprehensive Chemical Characterization Protocols

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Reagents used for synthesis were purchased from the corresponding reagent companies and used without further purification. Antibody used for Western blotting experiments were supplied by Abcam (U.K.), Cell Counting Kit-8 for cytotoxicity test (Dojindo, Japan). Cells were obtained from the Chinese Academy of Sciences (CAS), culture medium and serum (Thermo Fisher Scientific, USA). Mass spectra (MS) were measured on a Bruker Daltonics Esquire 6000 spectrometer. Recording all pH buffers by using a pHS-3E digital pH meter. UV–vis absorption spectra were measured by an AVARIAN UV-Cary100 spectrophotometer. Fluorescence emission spectra were measured using a Cary Eclipse fluorescence spectrophotometer. LSM 710 inverted fluorescence microscope (Carl Zeiss, Germany) was used for cell imaging. Fluorescent photographs of the samples were taken under a UV lamp at 365 nm. ImageJ software (USA) was used for cell fluorescence quantification and analysis of protein immunoblot results.
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2

Characterization of Phytochemical Compounds

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Column chromatography was conducted using silica gel (SiO2, 200–300 µm mesh; Qingdao Haiyang Chemical Co., Ltd., Qingdao, China) and Sephadex LH-20 (20–100 µm; Pharmacia, Uppsala, Sweden) as packing materials. Silica GF254 (10–40 mm) for TLC was supplied by the Qingdao Marine Chemical Factory, Qingdao, China. All TLC spots were visualized under UV light (254 nm) and stained with a 10% H2SO4 solution in ethanol, followed by heating. Optical rotations were recorded with a 341 polarimeter (Perkin-Elmer, Waltham, MA, USA) in a 1 dm cell. The UV spectra were measured on a UV-260 spectrophotometer (Shimadzu, Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto, Japan) and the IR spectra were obtained on a NEXUS 670 FT-IR spectrometer (Nicolet, Madison, WI, USA). Nuclear magnetic resonance spectra were recorded on INOVA-400 (Varian, Palo Alto, CA, USA) and AVANCE III-400 (Bruker, Billerica, MA, USA) spectrometers. Chemical shifts were given on a δ (ppm) scale using tetramethylsilane as the internal standard. High-resolution electrospray ionization (ESI) mass spectrometry (MS) was carried out on a APEX II mass spectrometer (Bruker Daltonics, Billerica, MA, USA) and ESI-MS spectra were determined on a Bruker Daltonics Esquire 6000 spectrometer. The ABTS and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) used were obtained from Aladdin Industrial Co. (Shanghai, China).
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