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3 protocols using vitexin

1

Bioactive Lipid Mediator Analysis

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Vasicine, vasicinone, isoorientin, isovitexin, vitexin, apigenin, 12,13-DiHOME, 9,10-DiHOME, 13(S)-HODE, 9(S)-HODE, 13-oxoODE, 9-oxoODE, 12,13-EpOME and 9,10-EpOME, 9-HpODE, and 7-ethoxyresorufin were purchased from Cayman Chemical (Ann Arbor, MI, USA). AITC, ionomycin calcium salt, HC-030039, and A967079 were from Sigma-Aldrich (St. Louis, MO, USA). DMSO was from Fisher Scientific (Waltham, MA, USA).
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2

Nicotine and Vitexin Effects on Wistar Rats

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All animal work was approved by the Campbell University Institutional Animal Care and Use Committee (IACUC). Male Wistar rats, shipped from the Charles River facility in Morrisville, NC and housed in the Campbell University Animal facility, were provided with food and water ad libitum and kept on a 12 h light/dark cycle at approximately 21 °C for 2–3 weeks before beginning any treatments. For all experiments, rats were 45–65 days old and between 148 and 260 g. Prior to the treatments specified in each protocol below, each rat was drug-naïve. Injections of nicotine tartrate or its vehicle (water) were injected subcutaneously (s.c.) at 0.4 mg/kg nicotine base (Sigma Aldrich, St. Louis, MO) and 2 mL/kg. Intraperitoneal (i.p.) injections of 30 or 60 mg/kg vitexin (Cayman Chemical Company, Ann Arbor, MI; Item 15116, Batch: 0500575) or its vehicle (5% DMSO, 5% Tween-80, 90% saline) were given at 3 or 6 mL/kg, respectively.
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3

Quantifying Advanced Glycation Endproducts in Herbal Teas

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Herbal teas samples were purchased from a local market (Taipei, Taiwan). Glyoxal (40 % in aqueous solution), diacetyl (DA), quercetin and o-phenylenediamine were purchased from Alfa Aesar (Great Britain, UK). MethylGlyoxal (40 % in aqueous solution), sodium azide, BSA, glucose, sodium hydroxide, Folin-Ciocalteu reagent, sodium bicarbonate and lanthionine (LAN) were purchased from Sigma (St. Louis, MO, USA). Aspalathin and 3-DG were purchased from Biosynth Carbosynth (St. Gallen, Switzerland). Glucosone, 3-deoxygalactosone (3-DGal), orientin, isoorientin, vitexin, isovitexin, isoquercetin and rutin were purchased from Cayman (Ann Arbor, MI, USA). Nothofagin was purchased from Aobious (Gloucester, MA, USA). CML, CEL, MG-H1, G-H1, methylGlyoxal-lysine dimer (MOLD), Glyoxal-lysine dimer (GOLD) and furosine were purchased form Iris Biotech (Marktredwitz, Germany). Salts, sugar, canola oil, white flour and baking powder were purchased from a local supermarket (Taipei, Taiwan). All solvents used in this study are analytical or LC-MS grade and sourced from Sigma.
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