50 ml polypropylene tubes

Manufactured by Greiner

Sourced in Germany

The 50-mL polypropylene tubes are a versatile laboratory equipment item. They are made of polypropylene and have a capacity of 50 milliliters. These tubes are designed for general laboratory use.

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2 protocols using 50 ml polypropylene tubes

ETC Inhibitors Handling Protocol

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Chemicals and reagents are listed in Table S1. All of the experiments were performed in flat bottom 24-wells plates (Corning, Corning, NY, USA) or in flat bottom 96-wells plates (Greiner Bio-One, Kremsmuenster, Austria). Dilutions were made in 50-mL polypropylene tubes and 12-mL polystyrene tubes, both from Greiner Bio-One. ETC inhibitors were dissolved in DMSO (rotenone, myxothiazol, and piericidin A) or ethanol (antimycin A) to concentrations of 2.0 mM (rotenone), 2.5 mM (myxothiazol), 1.7 mM (piericidin A), and 45.6 mM (antimycin A). As ETC inhibitors in general and piericidin A in particular are extremely toxic to humans and animals, the chemicals were handled and stored with special precautions in accordance with institutional policies.
All the concentrations throughout the manuscript refer to the final concentration unless otherwise indicated.

de Haan L.R., Reiniers M.J., Reeskamp L.F., Belkouz A., Ao L., Cheng S., Ding B., van Golen R.F, & Heger M. (2022). Experimental Conditions That Influence the Utility of 2′7′-Dichlorodihydrofluorescein Diacetate (DCFH2-DA) as a Fluorogenic Biosensor for Mitochondrial Redox Status. Antioxidants, 11(8), 1424.

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Digitate Sinter Sampling in NZ Geothermal Fields

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Different subtypes of digitate sinter from 11 hydrothermal pools and discharge channels in active geothermal fields of the TVZ, New Zealand, were sampled in April 2018. Each sampling site consisted of five spatial replicates (0.5 to 1 m apart). Sampled geothermal features were Orange Spring, Te Kopia, Parariki Stream, Rotokawa, Tikitere and Whangapaoa Spring (Fig. 1 and Table 1).
Actively growing, digitate siliceous sinter samples, spicular (fine, wide, bulgy) or knobby in morphology, were collected aseptically, kept in sterile 50 mL polypropylene tubes (Greiner Bioone, Germany), transported on dry ice, and stored at -80°C until further processing. Water temperatures were recorded in situ using a COMARK Evolution IV9001 temperature probe (Norfolk, UK), and pH values were recorded using the portable sension™156 Multiparameter Meter (Hach Company, USA). Sinters were photographed, their dimensions measured and their macro-morphologies characterized based on criteria from Walter, Bauld & Brock (1976) and Walter, Grotzinger & Schopf (1992) .

Sriaporn C., Campbell K.A., Millan M., Ruff S.W., Van Kranendonk M.J, & Handley K.M. (2020). Stromatolitic digitate sinters form under wide-ranging physicochemical conditions with diverse hot spring microbial communities. Geobiology, 18(5).

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