The chemicals tetrachloroauric (III) acid trihydrate (HAuCl4·3H2O, ≥99.9%), hydroquinone (C6H6O2, >99%), sodium citrate tribasic dihydrate (Na3C6H5O7·2H2O, ≥99.0%), hexadecyltrimethylammonium bromide, CTAB (C19H42BrN, 99%), silver nitrate (AgNO3), and sodium borohydride (NaBH4, 95%) were purchased from Sigma-Aldrich (Burlington, MA, USA). The hydrochloric acid (HCl, 37%) and formic acid (CH2O2, 98%) was purchased from Fluka (St. Gallen, Germany), and ascorbic acid (C6H8O6) was acquired from Carlo Erba (Val-de-Reuil, France). Ammonium bicarbonate (NH4HCO3, ≥97.5%), iodoacetamide (ICH2CONH2, ≥98%), acetonitrile (H3CCN, ≥99.5%), and 1,4-dithiothreitol (C4H10O2S2, ≥98%) were purchased from VWR (Pennsylvania, USA). The modified porcine trypsin was provided from Thermo Fischer Scientific (Waltham, MA, USA). There was no further purification of the chemicals acquired.
Ascorbic acid
Manufactured by Carlo Erba
Sourced in Italy, France
Ascorbic acid is a chemical compound that functions as an essential nutrient and antioxidant. It is commonly known as vitamin C.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (3 mentions)
Deferoxamine mesylate, by Merck Group (2 mentions)
2h 8 aea, by Cayman Chemical (2 mentions)
2h 5 2 ag, by Cayman Chemical (2 mentions)
2h 2 oea, by Cayman Chemical (2 mentions)
Chloroform, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Potassium hydroxide, by Carlo Erba (2 mentions)
Hydrochloric acid (hcl), by Carlo Erba (2 mentions)
Acetonitrile, by Avantor (1 mentions)
Sodium citrate tribasic dihydrate, by Merck Group (1 mentions)
Ammonium bicarbonate, by Avantor (1 mentions)
Iodoacetamide, by Avantor (1 mentions)
Hydroquinone, by Merck Group (1 mentions)
Ctab c19h42brn, by Merck Group (1 mentions)
Tetrachloroauric 3 acid trihydrate, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Hexadecyltrimethylammonium bromide, by Merck Group (1 mentions)
Dialysis membrane, by Merck Group (1 mentions)
Heregulin β1, by Merck Group (1 mentions)
6 protocols using ascorbic acid
1
Synthesis and Characterization of Gold Nanoparticles
2
Biomaterials for Cell Culture Applications
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Polyvinyl alcohol (molecular weight (MW) 124,000–184,000 Da), 70% hydrochloric acid, acetonitrile, elementary bromine, perchloric acid, sodium bicarbonate, bovine pancreatic trypsin, poly-L-lysine, Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12, Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum, penicillin/streptomycin solution, gentamicin solution, Heregulin β1, Forskolin, ethylenediaminetetraacetic acid, [3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide] and dialysis membranes (cut-off 8000 Da) were purchased from Sigma-Aldrich (S. Louis, Missouri, USA). Potassium permanganate, ascorbic acid, acetic acid, 2-propanol, 100% ethanol, sodium iodide, sodium cyanoborohydride and dimethyl sulfoxide were purchased from Carlo Erba (Milan, Italy).
3
Quantification of Endocannabinoids by HPLC
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The acetonitrile, methanol, chloroform, n-hexane, ethanol, acetic acid, and fatty acids standards were HPLC grade, and like deferoxamine mesylate, were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Ascorbic acid, potassium hydroxide, and hydrochloric acid were purchased from Carlo Erba (Milano, Italy). Internal deuterated standards for the AEA, 2-AG, and OEA quantification by isotope dilution ([2H]8 AEA, [2H]5 2-AG, [2H]2 OEA) were purchased from Cayman Chemicals (MI, USA).
4
Synthesis of Silica-Coated Paraquat Particles
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Ferrous sulfate heptahydrate (FeSO4·7H2O) (>99%) and ethanol (CH3CH2OH) (>99%) were obtained from Panreac (Barcelona, Spain). Paraquat dichloride (C12H14Cl2N2, >98%), starch (from rice), Tetraethyl orthosilicate (Si(OC2H5)4, TEOS, >99%), potassium nitrate (KNO3) (>99%), and 3-(triethoxysilyl)propyl isocyanate ((C2H5O)3Si(CH2)3NCO, ICPTES, 95%) were purchased from Sigma-Aldrich (Steinheim, Germany). Milli-Q water was obtained from the Synergy equipment from Millipore with a 0.22 μm filter (Darmstadt, Germany). Ammonia solution (25% NH3) was purchased from Riedel-de-Häen (Hanover, Germany) and potassium hydroxide (KOH) (>86%) was purchased from Pronolab (Lisbon, Portugal). N,N-Dimethylformamide (HCON(CH3)2) and ascorbic acid (>99%) were obtained from Carlo Erba Reagents (Peypin, France). Methanol (CH3OH) (>99%) was purchased from VWR International (Fontenay-sous-Bois, France) and κ-Carrageenan (300,000 g/mol) was obtained from Fluka Chemie (Steinheim, Germany). Unless clearly stated, all chemicals were used without any further treatment.
5
Phosphate Quantification and CSH Synthesis
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Disodium hydrogen phosphate (Na2HPO4) purchased from Merck (Darmstadt, Germany) was used to prepare a phosphate stock solution (100 mg L−1). All standard solutions and synthetic water containing phosphate were prepared by diluting a phosphate stock solution with ultrapure water generated by a water purification system (Merck, Darmstadt, Germany). ascorbic acid (C6H8O6, Fisher Scientific, Leicestershire, UK), ammonium heptamolybdate ((NH4)6Mo7O24·4H2O, Carlo Erba, Val-de-Reuil, France), and potassium antimonyl tartrate trihydrate (C8H4K2O12Sb2·3H2O, Carlo Erba, Val-de-Reuil, France) were used for phosphate quantification using the standard ascorbic acid method [30 ]. Calcium chloride (CaCl2, 99%, Loba, Mumbai, India) and sodium silicate (Na2SiO3, Loba, Mumbai, India) were used for CSH preparation. Rice flour (Erawan Brand, Nakhon Pathom, Thailand), tapioca starch (Jaydee Brand, Nakhon Pathom, Thailand), and red lime (food-grade, purchased from a local market in Kathu, Phuket, Thailand) were used for the synthesis of the monolithic cryogel column.
6
Quantitative Analysis of Fatty Acids and Endocannabinoids
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The acetonitrile, methanol, chloroform, n-hexane, ethanol, acetic acid, FA standards, all HPLC/MS grade, and deferoxamine mesylate were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Ascorbic acid, potassium hydroxide, and hydrochloric acid were purchased from Carlo Erba (Milano, Italy). FA standards including linoleic acid (18:2-n6), 20:4-n6, docosatetraenoic acid (22:4-n6), α-linolenic acid (18:3-n3), eicosapentaenoic acid (20:5-n3), docosahexaenoic acid (22:6-n3), palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0), and oleic acid (18:1-n9) were obtained from Sigma (Milan, Italy). Internal deuterated standards for the AEA, 2-AG, and OEA quantification by isotope dilution ([2H]8 AEA, [2H]5 2-AG, [2H]2 OEA, N-palmitoylethanolamine [2H]4 PEA) were purchased from Cayman Chemicals (Ann Arbor, MI, USA).
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