Accu chek aviva nano blood glucose meter

OGTT was performed on day 8 of experiment 1. The rats were subjected to 16 h of fasting and then orally administrated a glucose bolus (3 g/kg body weight). Blood samples were obtained from the tail vein at 0, 15, 30, 45, 60, 75, 90, 120, 180, and 240 min after the glucose load. Blood glucose concentrations were measured using an Accu-Chek^® Aviva Nano blood glucose meter (Roche Diagnostics, Tokyo, Japan). Blood samples at each time point were collected in heparinized microhematocrit capillaries (HIRSCHMANN^®; Hirschmann Laborgeräte GMbH & Co., Eberstadt, Germany) and centrifuged at 1,750 × g and room temperature for 15 min to obtain the plasma. Plasma insulin levels were measured with the Rat Insulin ELISA kit (Shibayagi, Gunma, Japan). Area under the curve (AUC) and incremental area under the curve (iAUC) were calculated from the glucose and insulin levels at each point.