Dilution buffer

Manufactured by BioXCell

Sourced in United States

Dilution buffer is a laboratory reagent used to prepare dilutions of samples, such as proteins, enzymes, or other biomolecules, prior to analysis or further processing. Its core function is to provide a controlled and consistent environment for diluting the sample, maintaining its stability and properties.

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Lab products found in correlation

Ruxolitinib incb18424, by MedChemExpress (1 mentions) Interferon gamma ifn γ, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

InVivoPure dilution buffer InVivoPure pH 6.5 Dilution Buffer PH 7.0 dilution buffer InVivoPure pH 7.0 Dilution Buffer

2 protocols using dilution buffer

Thrombopoietin Mimetic and Kinase Inhibitor Assay

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Ulixertinib (BVD-523, cat# HY-15816) and Ruxolitinib (INCB18424, cat# HY-50856) were purchased by MedChemExpress (Monmouth Junction, NJ, USA).
The thrombopoietin mimetic Romiplostim (Nplate^®) was kindly provided by Amgen (Thousand Oaks, CA, USA).
Anti-mouse OPN antibody (cat#BE0373, clone 103D6), the isotype control antibody (cat#BE0366, clone DV5-1) and the dilution buffer (cat#IP0070) were purchased by Bio X Cell (Lebanon, NH, USA).

Bianchi E., Rontauroli S., Tavernari L., Mirabile M., Pedrazzi F., Genovese E., Sartini S., Dall’Ora M., Grisendi G., Fabbiani L., Maccaferri M., Carretta C., Parenti S., Fantini S., Bartalucci N., Calabresi L., Balliu M., Guglielmelli P., Potenza L., Tagliafico E., Losi L., Dominici M., Luppi M., Vannucchi A.M, & Manfredini R. (2023). Inhibition of ERK1/2 signaling prevents bone marrow fibrosis by reducing osteopontin plasma levels in a myelofibrosis mouse model. Leukemia, 37(5), 1068-1079.

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Preparation of Chlorogenic Acid and IFN-γ Solutions

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Chlorogenic acid (CGA) was acquired from the Jiuzhang Biochemical Engineering Science and Technology Development Co., Ltd. (Chengdu, Sichuan, China), which with a purity of more than 99%. CGA was dissolved in normal saline (NS) at a concentration of 100 mM as a stock solution for in vitro experiment. Interferon-Gamma (IFN-γ) (PeproTech #315-05) was dissolved in 0.3% BSA solution at a concentration of 10 μg/mL as a stock solution. PD-1 neutralizing antibody and isotype control antibody were purchased from BioXcell, and diluted at a concentration of 2 mg / ml using Dilution Buffer (BioXcell).

Li R., Zhan Y., Ding X., Cui J., Han Y., Zhang J., Zhang J., Li W., Wang L, & Jiang J. (2024). Cancer Differentiation Inducer Chlorogenic Acid Suppresses PD-L1 Expression and Boosts Antitumor Immunity of PD-1 Antibody. International Journal of Biological Sciences, 20(1), 61-77.

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