Mouse intestinal organoids were cultured in mouse IntesiCultTM Organoid Growth Medium (STEMCELL Technologies, Cambridge, MA) by following the manufacturer’s instruction. 20 cm of small intestine proximal to stomach were harvested for culturing intestinal organoids, as this region allows optimal efficiency for generating organoids (Zietek et al., 2015 (link)). Organoids were used for experiments after two passages from primary culture or frozen.
Intesicult organoid growth medium
Manufactured by STEMCELL
IntesiCultTM Organoid Growth Medium is a cell culture medium formulated to support the growth and expansion of organoids derived from various tissue types, including intestinal, liver, kidney, and pancreatic organoids. The medium is designed to maintain the viability and differentiation potential of organoid cultures.
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Lab products found in correlation
2 protocols using intesicult organoid growth medium
1
Intestinal Organoid Culture Protocol
2
Intestinal Organoid Culture Protocol
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Mouse intestinal organoids were cultured in mouse IntesiCultTM Organoid Growth Medium (STEMCELL Technologies, Cambridge, MA) by following the manufacturer’s instruction. 20 cm of small intestine proximal to stomach were harvested for culturing intestinal organoids, as this region allows optimal efficiency for generating organoids (Zietek et al., 2015 (link)). Organoids were used for experiments after two passages from primary culture or frozen.
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