Anti gcp6

DAPI was purchased from Sigma. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic solutions were purchased from HiMedia, Inc. (Mumbai, India). Mouse monoclonal anti-TACC3, mouse polyclonal anti-GCP4, rabbit polyclonal anti-GCP6, and anti-γ-tubulin were obtained from Abcam; mouse monoclonal anti-α-tubulin and mouse monoclonal anti-γ-tubulin were purchased from Sigma; mouse monoclonal anti-GCP3, anti-GCP6 and goat polyclonal pericentrin antibody were obtained from Santa Cruz Biotechnology, Inc.; mouse monoclonal anti-actin was purchased from BD Biosciences; and rabbit polyclonal phospho-TACC3 antibody was purchased from Cell Signalling, USA. Rabbit polyclonal chTOG antibody was obtained from Abcam. The secondary antibodies, anti-mouse FITC, anti-rabbit TRITC, anti-mouse Cy5, anti-goat and anti-rabbit Cy5 were obtained from Jackson ImmunoResearch.

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic solutions were purchased from HiMedia, Inc. (Mumbai, India). Lipofectamine RNAiMAX, Lipofectamine 3000, and pre-stained protein ladder, 10 to 250 kDa were purchased from Thermo Fisher Scientific, U.S.A. PEI MAX- transfection agent from Polysciences. Mouse monoclonal anti-α-tubulin was purchased from Sigma (Cat # T5168); Mouse monoclonal anti-γ-tubulin for WB & IF (Cat # sc-17787), mouse monoclonal anti-TACC3 (Cat # sc-376883), mouse polyclonal anti-GCP4 (Cat # sc-271876), mouse monoclonal anti-GCP3 (Cat # sc-373758), anti-GCP6 (Cat # sc-374063), and mouse monoclonal anti-GFP antibody (Cat # sc-9996) were obtained from Santa Cruz Biotechnology, Inc.; rabbit polyclonal anti-ch-TOG antibody was obtained from Abcam (Cat # ab-236981); mouse monoclonal anti-actin was purchased from BD Biosciences (Cat # 612656); and rabbit monoclonal phospho-TACC3 antibody was purchased from Cell Signalling, U.S.A. (Cat # 8842). The secondary antibodies, anti-rabbit Alexa 568 and anti-mouse Cy5 were obtained from Jackson ImmunoResearch. DAPI was purchased from Sigma. GFP-Trap agarose beads used for IP were from Chromotech. Agarose A/G beads used for IP were from Santa Cruz Biotechnology, Inc.