FITC-labelled anti-CD3, PerCP-anti-CD4, BV785-anti-CD8, PECy7-anti-IFN-γ, BV421-anti-Fas ligand (FasL), and PE-anti-IL-17A monoclonal antibodies (mAbs) were purchased from BioLegend (San Diego, CA, USA). The PepTivator® SARS-CoV-2 Prot_S Complete Pool, SARS-CoV-2 Prot_S B.1.1.7 Mutation Pool, SARS-CoV-2 Prot_S B.1.1.7 WT Reference Pool, SARS-CoV-2 Prot_S B.1.351 Mutation Pool, SARS-CoV-2 Prot_S B.1.351 WT Reference Pool, SARS-CoV-2 Prot_S B.1.617.2 Mutation Pool and SARS-CoV-2 Prot_S B.1.617.2 WT Reference Pool were obtained from Miltenyi Biotec (Bergisch Gladbach, Germany). Brefeldin A and monensin were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Pecy7 anti ifn γ
Manufactured by BioLegend
Sourced in United States
PECy7-anti-IFN-γ is a fluorochrome-conjugated monoclonal antibody that binds to interferon-gamma (IFN-γ), a key cytokine involved in various immune responses. This product is intended for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Monensin, by Merck Group (1 mentions)
Anti cd4 percp, by BioLegend (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Brilliant violet 510 anti cd8a, by BioLegend (1 mentions)
Apc cy7 anti tcrβ, by BioLegend (1 mentions)
Anti cd4 pe, by BioLegend (1 mentions)
Percp cy5.5 anti cd44, by BioLegend (1 mentions)
Golgistop, by BD (1 mentions)
2 protocols using pecy7 anti ifn γ
1
Multicolor Flow Cytometry Analysis of SARS-CoV-2 Spike Protein
2
Comprehensive Cytokine Profiling of Activated Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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2 × 106 lymphocytes from spleens, thymuses and LNs were plated in round-bottomed 96-well plates in 1 ml RPMI complete media containing PMA (50 ng/ml, Sigma, P1585-1 MG), GolgiStop (1:1000, BD Biosciences, 554,724) and ionomycin (1 μM, CST, 9995S). After culturing for 5 h in 37 °C, 5% CO2, cells were collected and stained with PE-anti-CD4 (Biolegend, 100,408), Brilliant Violet 510-anti-CD8a (Biolegend, 100,751), PerCP/Cy5.5-anti-CD44 (Biolegend, 103,032), APC/Cy7-anti-TCRβ (Biolegend, 109,220). Then cells were fixed, permeabilized and stained with APC-anti-IL-4 (Biolegend, 504,106), PE/CY7-anti–IFN–γ (Biolegend, 505,826) and Brilliant Violet 421-anti-IL-17A (Biolegend, 506,925).
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