Rho associated protein kinase inhibitor

Manufactured by Selleck Chemicals

Rho-associated protein kinase inhibitor is a laboratory reagent that inhibits the activity of Rho-associated protein kinases (ROCK). ROCK enzymes play a role in regulating cellular processes such as cell migration, proliferation, and contraction. This inhibitor can be used to study the biological functions of ROCK in various in vitro research applications.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (2 mentions) Stemflex medium, by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) B 27 supplement without vitamin a, by Thermo Fisher Scientific (1 mentions) Mem neaa, by Merck Group (1 mentions) N2 supplement, by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Heparin, by Merck Group (1 mentions)

Close spelling variants of this product

Kinase inhibitors

2 protocols using rho associated protein kinase inhibitor

Differentiation of hiPSCs into Motor Neurons

Cited 2 times

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Healthy hiPSCs were procured from Coriell (ND03719, ND05280, ND00184, ND03231) and differentiated into motor neurons following published protocols.^12,85 (link) Briefly, hiPSCs were treated with small molecule cocktails that included CHIR99021, DMH1, and SB431542 to differentiate into OLIG2-positive motor neuron progenitors. These progenitors were removed from the culture surface with Accutase and seeded into non-tissue culture treated polystyrene dishes to form spheroidal aggregates. Cultures were further enriched through Notch inhibition (Compound E) and Hedgehog activation (purmorphamine). Motor neuron spheroids were then broken apart into small aggregates by slowly dissociating with a P1000 pipette until no chunks remained visible by eye. A homogenous sample of these aggregates was taken and further dissociated to obtain a reliable cell count. Finally, motor neurons were seeded dropwise at a density of roughly 750 000 neurons per substrate on top of myotubes undergoing differentiation for two to three days. All co-cultures were maintained in chick differentiation media supplemented with 10 μm Rho-associated protein kinase inhibitor (Selleck, S1049), which was removed after one day, and 10 ng/ml BDNF, CNTF, and GDNF, which were removed after one week.^24,85 (link) Media was refreshed every other day.

Santoso J.W., Li X., Gupta D., Suh G.C., Hendricks E., Lin S., Perry S., Ichida J.K., Dickman D, & McCain M.L. (2021). Engineering skeletal muscle tissues with advanced maturity improves synapse formation with human induced pluripotent stem cell-derived motor neurons. APL Bioengineering, 5(3), 036101.

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Cerebral Organoids from Human iPSCs

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Cerebral organoids were generated according to a previously published protocol (Lancaster and Knoblich, 2014) (link). In brief, on day 0, 4,500 human iPSCs were plated in each well of an ultra-low binding 96 well plates in StemFlex Medium containing 4ng/mL basic fibroblast growth factor (Thermo Fisher Scientific) and 50 mM Rho-associated protein kinase inhibitor (Selleckchem). Media was changed every other day for 6 days, then transferred to low adhesion 24 well plates in neural induction media (DMEM/F12, 1:100 N2 supplement (Thermo Fischer Scientific), Glutamax, MEM-NEAA, and 1 mg/mL heparin (Merck). Media was changed every other day for 5 days. On day 11, embryoid bodies were transferred to droplets of Matrigel and cultured in differentiation medium containing 1:1 mixture of DMEM/F12 and Neurobasal, 1:200 N2 supplement, 1:100 B27 supplement without vitamin A (Thermo Fisher Scientific), 3.5 mL/L 2-mercaptoethanol (Thermo Fisher Scientific), 1:4000 insulin (Sigma), 1:100 Glutamax, and 1:200 MEM-NEAA. After 4 days of stationary growth, plates were placed on a rotating platform with the same media as above except B27 supplement with vitamin A was used. Cells were cultured for 90 days with media changes every 2 days.
Cell Reports 39, 110643, April 5, 2022 e6

Huang C.Y., Nicholson M.W., Wang J.Y., Ting C.Y., Tsai M.H., Cheng Y.C., Liu C.L., Chan D.Z.H., Lee Y.C., Hsu C.C., Hsu Y.H., Yang C.F., Chang C.M.C., Ruan S.C., Lin P.J., Lin J.H., Chen L.L., Hsieh M.L., Cheng Y.Y., Hsu W.T., Lin Y.L., Chen C.H., Hsu Y.H., Wu Y.T., Hacker T.A., Wu J.C., Kamp T.J, & Hsieh P.C.H. (2022). Population-based high-throughput toxicity screen of human iPSC-derived cardiomyocytes and neurons. Cell reports, 39(1).

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