Pcdna3.1 n ha vector

Manufactured by Thermo Fisher Scientific

The PcDNA3.1 N-HA vector is a plasmid used for the expression of recombinant proteins in mammalian cell lines. It contains a human cytomegalovirus (CMV) immediate-early promoter for high-level expression of the target gene, as well as an N-terminal hemagglutinin (HA) tag for detection and purification of the expressed protein.

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Lab products found in correlation

Acgfp c1 vector, by Addgene (2 mentions) Pcdna5 frt plasmid, by Thermo Fisher Scientific (2 mentions) Pcdna5 frt vector, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PcDNA3.1+HA vector PcDNA3-HA vector PcDNA3.1-HA PcDNA3.1 vector (vector) PcDNA3.1 vector PcDNA3-HA PcDNA3 vector PcDNA3.1 (þ) PcDNA3.1

2 protocols using pcdna3.1 n ha vector

Cloning and Expression of Protein Constructs

Cited 1 time

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3xFLAG-tagged SANS full length and deletion constructs were designed and cloned in house as previously described (29 (link)). HA-PRPF31 constructs were kind gifts from Dr. Utz Fischer (41 (link)). Deletion constructs of PRPF31 (N-term (aa1–165), C-term (aa 166–499)) were produced by PCR from the HA-PRPF31 plasmid and cloned into the pcDNA3.1 N-HA vector (Thermo Fisher). Deletion constructs of PRPF6 (NTD (aa 1–126), HAT1-6 (aa 293–592), HAT7-13 (aa 593–941)) were cloned into AcGFP-C1 vector (Addgene: 64607). RON (MST1R) minigene was a kind gift from Dr. Julian Koenig (IMB-Mainz), and USH1C minigene was amplified from the genomic DNA of HEK293T cells and cloned into the pcDNA5/FRT vector (Thermo Fisher). The pcDNA5/FRT-PRPF4 plasmid was constructed by cloning the PRPF4 gene fused to an N-terminal FLAG/HA tag into the pcDNA5/FRT plasmid (Thermo Fisher).

Yildirim A., Mozaffari-Jovin S., Wallisch A.K., Schäfer J., Ludwig S.E., Urlaub H., Lührmann R, & Wolfrum U. (2021). SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes. Nucleic Acids Research, 49(10), 5845-5866.

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Molecular Cloning of Protein Constructs

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3xFLAG-tagged SANS full length and deletion constructs were designed and cloned in house as previously described (29) . HA-PRPF31 constructs were kind gifts from Dr. Utz Fischer (University of Wuerzburg, Germany). Deletion constructs of PRPF31 (N-term (aa1-165), Cterm (aa 166-499)) were produced by PCR from the HA-PRPF31 plasmid and cloned into the pcDNA3.1 N-HA vector (Thermo Fisher). Deletion constructs of PRPF6 (NTD (aa 1-126), HAT1-6 (aa 293-592), HAT7-13 (aa 593-941)) were cloned into AcGFP-C1 vector (Addgene: 64607). RON (MST1R) minigene was a kind gift from Dr. Julian Koenig (IMB-Mainz), and USH1C minigene was amplified from the genomic DNA of HEK293T cells and cloned into the pcDNA5/FRT vector (Thermo Fisher). The pcDNA5/FRT-PRPF4 plasmid was constructed by cloning the PRPF4 gene fused to an N-terminal FLAG/HA tag into the pcDNA5/FRT plasmid (Thermo Fisher).

Yıldırım A., Mozaffari‐Jovin S., Wallisch A., Ries J., Ludwig S., Urlaub H., Lührmann R., & Wolfrum U. (2020). SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes.

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