All reagents and solvents used during the extraction were analytical grade. Strains were incubated in 2216E medium (pH 7.4 ~ 7.6), which was composed of 5.0 g/L peptone, 1.0 g/L yeast extract, 0.1 g/L FeC6H5O7, 19.45 g/L NaCl, 5.98 g/L MgCl2, 3.24 g/L Na2SO4, 1.8 g/L CaCl2, 0.55 g/L KCl, 0.16 g/L Na2CO3, 0.08 g/L KBr, 0.034 g/L SrCl2, 0.022 g/L H3BO3, 0.004 g/L Na2SiO3, 0.0024 g/L NaF, 0.0016 g/L NH4NO3 and 0.008 g/L Na2HPO3. Lysozyme (Solarbio Science & Technology, Beijing, China) was used for cell wall digestion. Tris-HCl buffer (10 mM, pH 7.4), chloroform-methanol (2:1 v/v) and hexane-diethyl ether (85:15, v/v) were used for MK extraction. These organic reagents were purchased from Xilong Scientific Co., Ltd., Guangzhou, China. The MK-9 standard was purchased from GLPBIO, Montclair, CA, USA. Mass spectrometry-grade methanol (Sigma-Aldrich, St. Louis, MO, USA) and isopropanol (Fisher Chemical, Thermo-Fisher Scientific Inc.) were used for high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC), and mass spectrometer (MS).
Mass spectrometry grade methanol
Manufactured by Merck Group
Sourced in United States, Germany
Mass spectrometry-grade methanol is a highly purified form of methanol designed for use in mass spectrometry applications. It is a colorless, volatile liquid with a characteristic alcoholic odor. The product meets stringent purity requirements to ensure consistent and reliable performance in sensitive analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (4 mentions)
Formic acid, by Merck Group (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
Lysozyme, by Solarbio (1 mentions)
Fluconazole, by LGC (1 mentions)
Milli q system, by Merck Group (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
Orbitrap exploris 120, by Thermo Fisher Scientific (1 mentions)
Acquity uplc beh amide column, by Waters Corporation (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Protease inhibitor cocktail tablet, by Roche (1 mentions)
Pfhxa, by Wellington Laboratories (1 mentions)
Pfhxs, by Wellington Laboratories (1 mentions)
Pfhpa, by Wellington Laboratories (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Fatty acid methyl ester standard, by Merck Group (1 mentions)
Diatomaceous earth, by Merck Group (1 mentions)
Meiqx, by LGC (1 mentions)
4 8 dimeiqx, by LGC (1 mentions)
Amino acid standard, by Merck Group (1 mentions)
7 protocols using mass spectrometry grade methanol
1
Extraction and Identification of Menaquinones
2
Fluconazole Quantification via Mass Spectrometry
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Fluconazole (> 99.0%) and its internal standard (IS, Fluconazole-d4 stable isotope (> 99.0%)) were purchased from Toronto Research Chemical (North York, Ontario, Canada). Mass spectrometry-grade methanol, acetonitrile, formic acid, and ammonium formate were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultra-pure water was obtained using a Milli-Q system (Millipore, Bedford, USA). Stock solutions of Fluconazole (2 mg/mL) and IS (1 mg/mL) were prepared in acetonitrile and stored at − 80 °C in a freezer.
3
Metabolomics Profiling using UPLC-Orbitrap
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Mass spectrometry grade methanol, ammonium acetate, acetonitrile, and ammonium hydroxide were from Sigma Aldrich. ACQUITY UPLC BEH Amide column (1.7 μm, 2.1 mm× 100 mm, Waters Corporation, Milford, MA, USA) was used for separation. Vanquish liquid chromatography system coupled with Orbitrap Exploris 120 (Thermo Fisher Scientific) was used for metabolomics profiling.
4
Optic Nerve Lipid and Protein Extraction
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Single optic nerves were thawed, homogenized via bath sonication and sequential freeze-thaw cycles in dry ice in high salt PBS (1M NaCl) supplemented with a protease-inhibitor cocktail tablet (Roche Diagnostics Corporation, Indianapolis, IN). Phospholipid internal standards dissolved in methanol di-14:0 PE (phosphatidylethanolamine), di-14:0 PC (phosphatidylcholine), 17:0 SM (sphingomylein), 14:0 LPE (lysophosphatidylethanolamine), 14:0 LPC (lusophosphatidylcholine), and di-16:0 PI (phosphatidylinositol) (Materya LLC, Pleasant Gap, PA) were added. The homogenates were then clarified by centrifugation for 15 minutes at 20,000 x g RCF at 4°C. Proteins were precipitated in both the resultant supernatant and pellet by addition of 7 volumes of chilled mass-spectrometry grade methanol (Sigma-Aldrich, St Louis, MO). The methanolic supernatants were combined in a new tube, and the pellets were then extracted with 200 μl of 2:1 chloroform:MeOH to extract lipids. The MeOH and chloroform-MeOH extracts were combined and used for lipid analysis, and the pellets were used for proteomic analyses.
5
PFAS Adsorption by Chloride Resins
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In this study, ten target PFASs (PFBA, PFBS, PFHxA, PFHxS, PFHpA, PFOA, PFOS, PFDA, GenX, and 6:2 FTSA) were purchased from Wellington Laboratories (Guelph, ON, Canada), with their detailed information shown in Tables S1 and S2 in Supplementary Materials (SM) . Four types of chloride resins (HPR4700, WA10, S6368, and A111S) were selected. Specifically, HPR4700 (DuPont, Wilmington, NC, USA), WA10 (Mitsubishi Chemical, Tokyo, Japan), S6368 (Lanxess, Cologne, Germany), and A111S (Purolite, King of Prussia, PA, USA) were gel-type strong base resin, gel-type weak base resin, macroreticular strong base resin, and macroreticular weak base resin, respectively. The physical morphology and property of these resins are provided in Figure S1 and Table S3 . Fulvic acid (95%) was obtained from Thermo Fisher (Scoresby, North Ryde, Australia). Guaranteed reagents (hydrochloric acid, sodium hydroxide, sodium sulfate, sodium carbonate, and sodium bicarbonate) were all bought from CNW (Shanghai, China). Ultrapure water was produced by the Milli-Q water purification system (Millipore, Boston, MA, USA). Mass spectrometry grade methanol was purchased from Merck (Burlington, MA, USA).
6
Comprehensive Analytical Protocol for Food Analysis
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HA standards, PhIP, MeIQx, and 4,8-DiMeIQx were purchased from Toronto Research Chemicals (Toronto, Canada). Oasis MCX cartridges (500 mg) were from Waters Inc. (Milford, MA, USA). Amino acid standards, fatty acid methyl ester standards, Bond-Elut reservoirs, diatomaceous earth, Folin–Ciocalteu reagent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tripyridyl-s-triazine (TPTZ), and quercetin were from Sigma-Aldrich (St Louis, MO, USA). Mass spectrometry grade methanol, acetonitrile, ammonium acetate, and formic acid were purchased from Merck KGaA (Darmstadt, Germany). Amino acid test kit (MSLAB-45+AA) was from Beijing Mass Spectrometry Medical Research Co., Ltd. (Beijing, China). Aluminum chloride (AlCl3), sodium carbonate and other general reagents were from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
7
Lipid Profiling of Biological Samples
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The reference standards of LPC(13:0) (internal standard, IS), LPC(14:0), LPC(16:0), LPC(17:0), LPC(18:1) and LPC(20:0) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Mass spectrometry grade-methanol and acetonitrile were obtained from Merck Inc. (Darmstadt, HE, Germany). Analytical reagent-chloroform and HPLC grade-formic acid were purchased from Xilong Technology Co., Ltd. (Shantou, China). Phosphate buffer saline (PBS) was supplied by Servicebio Technology Co., Ltd. (Wuhan, China). Bovine serum albumin (BSA) was provided by Energy Chemical Technology Co., Ltd. (Shanghai, China). CH and APAP were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Isoflurane was supplied by RWD Life Science CO., Ltd. (Shenzhen, China)
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