Trizma base tris

Manufactured by Merck Group

Trizma base (TRIS) is a chemical compound commonly used as a buffer in various laboratory applications. It is a zwitterionic organic compound that can regulate the pH of aqueous solutions. Trizma base is widely used in biochemistry, molecular biology, and cell culture protocols to maintain a stable pH environment for experiments and assays.

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4 protocols using trizma base tris

Fluorescent Lipid Probe Synthesis and Characterization

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All used chemicals were of analytical grade and purchased from Sigma-Aldrich (Denmark) unless otherwise stated. Alexa-488 mono-functional maleimide was purchased from Thermo Fisher Scientific, Denmark. Labeled phospholipids, 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-ATTO655 (DOPE-ATTO655) were purchased from ATTO-TEC GmbH, Siegen, Germany. 1,2-Di-O-lauryl-rac-glycero-3-(glutaric acid 6-methylresorufin ester) (lipidated resorufin) (Sigma-Aldrich, CAS: 195833-46-6), glyceryl trimyristate (trimyristin) (Sigma-Aldrich, CAS: 555-45-3), Trizma base (TRIS) (Sigma-Aldrich, CAS: 77-86-1), n-hexane (Sigma-Aldrich, CAS: 110-54-3), 96% ethanol (Alere, CAS: 64-17-5), microtiter plates (ThermoFisher, Cat. # 237107).

Bohr S.S., Lund P.M., Kallenbach A.S., Pinholt H., Thomsen J., Iversen L., Svendsen A., Christensen S.M, & Hatzakis N.S. (2019). Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition. Scientific Reports, 9, 16169.

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Benzo[a]pyrene Metabolic Assay

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3-OHBaP was ordered from Toronto Research Chemicals (TRC) Canada (North York, Canada). 3’-phosphate 5’-phosphosulfate (PAPS) lithium salt was purchased from Santa Cruz Biotechnology (Dallas, Texas, United States), BaP, nicotinamide adenine dinucleotide phosphate (NADPH), sodium salt, sodium phosphate, sodium chloride and Trizma^® base (TRIS) were purchased at Sigma-Aldrich (Zwijndrecht, The Netherlands). Uridine 5’-diphosphoglucuronic acid (UDPGA) trisodium salt was purchased from Carbosynth (Compton, United Kingdom). Pooled liver and lung S9 fractions and microsomes from male Sprague–Dawley (SD) rats were ordered from Tebu-Bio (Heerhugowaard, The Netherlands). Acetonitrile (ACN) was purchased from Biosolve (Dieuze, France). Dimethyl sulfoxide (DMSO) was obtained from Acros Organics (Geel, Belgium). Potassium hydrogen phosphate (K₂HPO₄) and trifluoroacetic acid (TFA) were purchased from Merck (Darmstadt, Germany).

Wang D., Rietdijk M.H., Kamelia L., Boogaard P.J, & Rietjens I.M. (2021). Predicting the in vivo developmental toxicity of benzo[a]pyrene (BaP) in rats by an in vitro–in silico approach. Archives of Toxicology, 95(10), 3323-3340.

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Oligonucleotide Synthesis and Purification

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Custom DNA oligonucleotides were purchased from MilliporeSigma with HPLC purification and all sequences are listed in Fig. S1.† Trizma® (Tris) base, hydrochloric acid (HCl), and magnesium chloride (MgCl₂) were obtained from Sigma-Aldrich. Sodium chloride (NaCl) and phosphate buffered saline (PBS) were purchased from Fisher Scientific. Water was obtained from a Milli-Q purification system from Milli-poreSigma. Aqueous mobile phases were filtered through 0.22 μm polyethersulfone (PES) membrane filters (Fisher Scientific) and used within two weeks.

Langlois N.I, & Clark H.A. (2022). Characterization of DNA nanostructure stability by size exclusion chromatography. Analytical methods : advancing methods and applications, 14(10), 1006-1014.

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Quantitative SIL Peptide Preparation

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Synthetic
stable isotope-labeled (SIL) peptides (SpikeTides_TQL) that are isotopically
labeled and quantified (Table 2) were obtained from JPT Peptide Technologies GmbH (Berlin,
Germany). Guanidine hydrochloride, sodium hydroxide, dithiothreitol
(DTT), ammonium hydrocarbonate, iodoacetamide, chloroform, methanol,
MS-grade ACN, formic acid, sodium-EDTA, Trizma (Tris base), and all
other chemicals and reagents were from Sigma (Saint Louis, MO). ProteaseMAX,
sequencing-grade lysyl endopeptidase LysC, TPCK-treated trypsin, and
urea were from Promega (Madison, WI). Solid phase extraction (SPE)
cartridges were Strata-X, 33 μm, polymeric reversed phase (Part
# 8B-S100-AAK) (10 mg/mL) purchased from Phenomenex (Torrance, CA).

Hammid A., Fallon J.K., Lassila T., Salluce G., Smith P.C., Tolonen A., Sauer A., Urtti A, & Honkakoski P. (2021). Carboxylesterase Activities and Protein Expression in Rabbit and Pig Ocular Tissues. Molecular Pharmaceutics, 18(3), 1305-1316.

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