Liver sections were incubated with primary antibodies, followed by incubation with biotinylated secondary antibodies. The following primary antibodies were used: anti-STK25 (YSK1; sc-6865; Santa Cruz Biotechnology, Santa Cruz, CA), anti-PCNA (MA5-1158; Invitrogen), anti-Ki67 (14-5698-82; Invitrogen), anti-Gr1 (Ly6C) (ab15627; Abcam, Cambridge, UK), anti-F4/80 (MCA497GA; Bio-Rad, Hercules, CA), anti–collagen I (SAB4500362; Sigma-Aldrich), anti–α-smooth muscle actin (ab5694; Abcam), anti–4-HNE (sc-130083; Santa Cruz Biotechnology), anti-PEX5 (PA5-58716; Invitrogen), and anti-ubiquitin (ab411; Abcam). For immunohistochemical detection, anti-goat IgG (E0466; Dako, Glostrup, Denmark) and anti-mouse IgG (E0464; Dako) secondary antibodies were used, followed by horseradish-peroxidase–conjugated streptavidin (P0397; Dako) and diaminobenzidine staining (K3467; Dako). For immunofluorescence detection, Alexa Fluor-594–labeled goat anti-rat IgG (A11007; Invitrogen), Alexa Fluor-488–labeled rabbit anti-mouse IgG (A11059; Invitrogen), Alexa Fluor-594–labeled donkey anti-goat IgG (A11058; Invitrogen), and Alexa Fluor-594–labeled donkey anti-rabbit IgG (A21207; Invitrogen) secondary antibodies were used. The stained area was quantified in 5 randomly selected microscopic fields (×200) per mouse using ImageJ software.
Alexa fluor 594 labeled goat anti rat igg
Manufactured by Thermo Fisher Scientific
Alexa Fluor-594–labeled goat anti-rat IgG is a secondary antibody conjugated with the Alexa Fluor 594 fluorescent dye. It is designed to detect and visualize rat immunoglobulin G (IgG) in various applications, such as immunohistochemistry, flow cytometry, and Western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Ab15627, by Abcam (1 mentions)
A11059, by Thermo Fisher Scientific (1 mentions)
Anti f4 80 mca497ga, by Bio-Rad (1 mentions)
E0466, by Agilent Technologies (1 mentions)
P0397, by Agilent Technologies (1 mentions)
A11058, by Thermo Fisher Scientific (1 mentions)
K3467, by Agilent Technologies (1 mentions)
E0464, by Agilent Technologies (1 mentions)
Ab5694, by Abcam (1 mentions)
Tcs sp5 confocal laser microscope, by Leica (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
2 protocols using alexa fluor 594 labeled goat anti rat igg
1
Immunohistochemical Analysis of Liver Markers
2
Localization of Pseudomonas ExoS Effector in Host Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine the cellular location of the effector protein ExoS, A549 cells grown on coverslips were challenged with bacteria (MOI = 50) for 2 h at 37 °C, 5% CO2. Immunostaining was performed with the rat anti-ExoS antibody (generated in our laboratory), followed by hybridization with Alexa Fluor 594-labeled goat anti-rat IgG (1:200, Invitrogen). The cellular nuclei were stained with 4, 6-diamidino-2-phenylindole (DAPI) (1:1000, Beyotime). Images were acquired and analyzed using a Leica TCS SP5 laser confocal microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!