Small molecules were added at the following concentrations and for the indicated pretreatment times unless otherwise indicated: ACQ090 (20 µM; Novartis; Bänziger et al., 2003 (link)), 3-isobutyl-1-methylxanthine (IBMX; 500 µM; 30 min preincubation; I5879; Sigma-Aldrich), L796,778 (10 µM; Merck; Rohrer et al., 1998 (link)), PKI (50 µM; 40 min preincubation; BML-P210-0500; Enzo Life Sciences), PTX (10 ng/ml; 16 h preincubation; 180; List Biological Laboratories), Rp-cAMPS (10 µM; 2 h preincubation; sc-24010; Santa Cruz Biotechnology, Inc.), SAG (200 nM; ALX-270-426-M001; Enzo Life Sciences), sst (10 µM; ASR-003; Alomone Labs), and SQ22536 (500 µM; 40 min preincubation; S153; Sigma-Aldrich).
Sc 24010
Manufactured by Santa Cruz Biotechnology
Sc-24010 is a lab equipment product from Santa Cruz Biotechnology. It is a centrifuge designed for general laboratory use. The core function of Sc-24010 is to separate different components of a liquid mixture based on their density differences through the application of centrifugal force.
Automatically generated - may contain errors
2 protocols using sc 24010
1
Small Molecule Pretreatment Assay
2
CXCL10 Expression Regulation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells grown to 80% confluency in 12-well dishes were starved overnight in media with 0.5% FBS, then treated overnight with AZD0530 (100 nM, LC Laboratories, Wobum, MA), bis-indolylmaleimide (500 nM; R&D Systems), LY294002 (50 μM, # 1130, R&D Systems), Rp-cAMPS (100 nM, sc-24010, Santa Cruz), VX680 (20 nM; gift of T. Ouchi, Roswell Park Cancer Institute), or PBS control, with or without treatment with IFNγ (100 ng/ml, R&D Systems). Media samples were then centrifuged at 2000 RPM for 5 min, and 100 μl taken for mouse-specific CXCL10-ELISA analysis (#DY466, R&D Systems). All experiments were done in triplicate, and repeated at least twice.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!