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Phospho p pkcα ser 657

Manufactured by Santa Cruz Biotechnology
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Phospho - p-PKCα (Ser 657) is a lab equipment product that detects the phosphorylation of protein kinase C alpha (PKCα) at serine 657. This product is used for research purposes to study the activity and regulation of PKCα, a key signaling enzyme involved in various cellular processes.

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Lab products found in correlation

Non phosphorylated pkc 63, by Santa Cruz Biotechnology (2 mentions)

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P-PKCα (5 citations)

2 protocols using phospho p pkcα ser 657

1

Quantification of Phosphorylated PKC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from tissue samples in RIPA buffer. A total of 30 or 20 μg of protein from each sample were separated by SDS-PAGE, and transferred onto nitrocellulose membranes. Membranes were incubated with specific primary antibodies, non-phosphorylated – PKC 63 (link) and phospho – p-PKCα (Ser 657) (diluted 1:1000) (Santa Cruz, USA).
Ganesh B.P., Hall A., Ayyaswamy S., Nelson J.W., Fultz R., Major A., Haag A., Esparza M., Lugo M., Venable S., Whary M., Fox J.G, & Versalovic J. (2017). Diacylglycerol kinase synthesized by commensal Lactobacillus reuteri diminishes Protein Kinase C phosphorylation and histamine-mediated signaling in the mammalian intestinal epithelium. Mucosal immunology, 11(2), 380-393.
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2

Quantification of Phosphorylated PKC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from tissue samples in RIPA buffer. A total of 30 or 20 μg of protein from each sample were separated by SDS-PAGE, and transferred onto nitrocellulose membranes. Membranes were incubated with specific primary antibodies, non-phosphorylated – PKC 63 (link) and phospho – p-PKCα (Ser 657) (diluted 1:1000) (Santa Cruz, USA).
Ganesh B.P., Hall A., Ayyaswamy S., Nelson J.W., Fultz R., Major A., Haag A., Esparza M., Lugo M., Venable S., Whary M., Fox J.G, & Versalovic J. (2017). Diacylglycerol kinase synthesized by commensal Lactobacillus reuteri diminishes Protein Kinase C phosphorylation and histamine-mediated signaling in the mammalian intestinal epithelium. Mucosal immunology, 11(2), 380-393.
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