Rabbit mab anti hsp90

Manufactured by Cell Signaling Technology

Rabbit mAb anti-HSP90 is a primary antibody that specifically binds to the heat shock protein 90 (HSP90) in various species. HSP90 is a molecular chaperone that plays a crucial role in the folding and stabilization of numerous client proteins.

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Lab products found in correlation

Rabbit anti phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Gcn2 inhibitor, by MedChemExpress (1 mentions) Mouse anti α tubulin mab, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Rneasy plus mini kit, by Qiagen (1 mentions) Rabbit anti cyclophilin b, by Thermo Fisher Scientific (1 mentions) Rabbit anti gm130, by Abcam (1 mentions) Guinea pig anti insulin, by Fortrea (1 mentions)

2 protocols using rabbit mab anti hsp90

Antibody Characterization for Insulin Detection

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Antibodies included guinea pig anti-insulin (Covance, RRID:AB_10013624), mouse mAb anti-rodent proinsulin (Novus Biologicals, RRID:AB_1107982), mouse mAb anti-human proinsulin B-C junction sequence KTRREAEDLQ (Abmart, RRID:AB_2921300), a custom-made rabbit polyclonal antibody against mouse C-peptide-2 (C-PQVAQLELGGGPGAGDLQT, Abmart, RRID:AB_2921302), mouse mAb anti-α-tubulin (Sigma-Aldrich, RRID:AB_477579), mouse mAb anti-beta actin (Proteintech, RRID:AB_2919667), rabbit mAb anti-HSP90 (Cell Signaling, RRID:AB_2233307), and rabbit anti-phospho-eIF2α (Ser51) (Cell Signaling, RRID:AB_330951). All tissue culture reagents were from Invitrogen. Dithiothreitol, MG132, and CHX were from Sigma. GCN2 inhibitor was from MedChemExpress (HY-112654). Mycolactone was isolated from Mycobacterium ulcerans, as described (49 ). RNeasy Plus Mini Kit was from Qiagen (cat. #74134). Anti-flag magnetic agarose beads were from Fisher Scientific.

Xu X., Arunagiri A., Alam M., Haataja L., Evans C.R., Zhao I., Castro-Gutierrez R., Russ H.A., Demangel C., Qi L., Tsai B., Liu M, & Arvan P. (2023). Nutrient-dependent regulation of β-cell proinsulin content. The Journal of Biological Chemistry, 299(7), 104836.

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Recombinant Proinsulin Detection Assay

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Chemicals were purchased from Sigma‐Aldrich or ThermoFisher. Recombinant human proinsulin (expressed in E. coli) was purchased from Creative Biomart (Catalog # INS‐315H). Antibodies in this study include rabbit anti‐Myc (Immunology Consultants, RRID:AB_2921297); guinea pig anti‐insulin (Covance, RRID:AB_10013624); rabbit anti‐cyclophilin B (ThermoFisher, RRID:AB_2169138); rabbit polyclonal anti‐calreticulin (Abcam RRID:AB_303402); rabbit mAb anti‐Hsp90 (Cell Signaling, RRID:AB_2233307); rabbit anti‐GM130 (Abcam, RRID:AB_880266); mouse mAb anti‐human proinsulin B‐C junction sequence PKTRREAEDLQVGQ (Abmart, RRID:AB_2921300); mouse mAb anti‐rat proinsulin (CCI‐17, Novus Biologicals RRID:AB_1107982); mouse anti‐rat C‐peptide CII‐29 (Advanced Immunochemical Inc., Catalog ID: 1‐CP‐r); mouse anti rat C‐Peptide I 6H1 (Biorad Catalog # MCA2857) and as a back‐up, a custom‐made rabbit polyclonal antibody against mouse C‐peptide‐2 sequence PQVAQLELGGGPGAGDLQT (Biogot, RRID:AB_2921302).

Arunagiri A., Alam M., Haataja L., Draz H., Alasad B., Samy P., Sadique N., Tong Y., Cai Y., Shakeri H., Fantuzzi F., Ibrahim H., Jang I., Sidarala V., Soleimanpour S.A., Satin L.S., Otonkoski T., Cnop M., Itkin‐Ansari P., Kaufman R.J., Liu M, & Arvan P. (2024). Proinsulin folding and trafficking defects trigger a common pathological disturbance of endoplasmic reticulum homeostasis. Protein Science : A Publication of the Protein Society, 33(4), e4949.

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