The GFP-neo-labelled C0 was used as nucleus donor (ρ0 cell) which was treated with rhodamine 6G (R-6G) (Sigma) at 5 μg/ml for 8 days29 (link). C1, C2 and C0 cells were enucleated as mitochondria donors (enucleated cytoplasts) by centrifugation in the presence of 10 μg/ml cytochalasin B (CB) (Sigma). A total of 106 ρ0 cells and 106 enucleated cytoplasts were fused with 45% polyethylene glycol (PEG) (MW 1450; Sigma), and selected by G418 medium with free of uridine and sodium pyruvate. Mitochondria were stained by 1/5000 w/v Janus Green B (Sigma) and nuclei by Giemsa working solution (AppliChem), and all cells were observed under microscopy at ×400 magnification. The procedure is outlined in Fig. 1 .
Janus green b
Manufactured by Merck Group
Sourced in United States
Janus Green B is a synthetic dye compound used as a staining agent in biological and biochemical applications. It is a dark green crystalline powder that is soluble in water and organic solvents. The primary function of Janus Green B is to serve as a vital stain, specifically for the mitochondria within cells.
Automatically generated - may contain errors
Lab products found in correlation
Cytochalasin b cb, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Paclitaxel, by Maclin Biochemical Technology (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Fluo 4 am, by Beyotime (1 mentions)
2 protocols using janus green b
1
Mitochondrial Transfer via Cytoplast Fusion
2
Multifunctional Nanocomposite Platform for Cancer Therapy
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PEDOT:PSS (Clevios PH 1000) was purchased from Wuhan Zhuojia Technology Co., Ltd. (China). Carbon nanotubes (SWCNTs-COOH, diameter 1–3 nm, and length 1–3 μm) were obtained from XFNANO Materials Tech Co., Ltd. (Jiangsu, China). Sodium carboxymethyl cellulose (CMC) and D (+)-glucose were bought from Aladdin Industrial Co., Ltd. (Shanghai, China). Paclitaxel was purchased from Shanghai Macklin Biochemical Co., Ltd. (China). Resveratrol and Janus green B were bought from Sigma-Aldrich (USA). Fluo-4 AM was obtained from Beyotime Biotechnology Co., Ltd. (China). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, penicillin and streptomycin for cell culture were obtained from GIBCO (USA). All the other chemicals and solvents of analytical grade were obtained from Sinopharm Chemical Reagent Co., Ltd. (China) and used as received unless stated otherwise.
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