Hrp conjugated anti mouse and anti goat igg

Dulbecco's modified eagle's medium (DMEM), LPS (Escherichia coli 0111:B4), FITC-dextran (40,000 Da), and glycyrrhizic acid (GA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). There was no detectable endotoxin (<0.10 endotoxin units/mL) in the GA samples, as determined by Endospecy. Recombinant moues IFN-γ, M-CSF, and IL-4 were obtained from PeproTech Inc. (Rocky Hill, NJ, USA). Anti-mouse antibodies FITC-F4/80, -CD80, -CD86, -MR and PE-MHCII, and -CCR7 as well as anti-NF-κB p65 were obtained from Biolegend (San Diego, CA, USA). The ELISA kits for TNF-α, IL-6, IL-10, and IL-12p70 were obtained from eBioscience (San Diego, CA, USA). Antibodies against β-actin, iNOS, phospho-JNK, JNK, phospho-p38 MAPK, p38 MAPK, IκBα, LaminB1, and HRP-conjugated anti-mouse and anti-goat IgG were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA), and phospho-ERK1/2, anti-ERK1/2, phospho-STAT1, and anti-STAT1 antibodies were obtained from BD Pharmingen (San Jose, CA, USA). Inhibitors BAY 11-7082, SP600125, SB203580, and U0126 were purchased from Beyotime Biotechnology (Haimen, Jiangsu, China).

GL was purchased from Sigma-Aldrich (Sigma-Aldrich, USA). Gentamycin (GM) was obtained from Amersco (Amersco, USA). LPS (Escherichia coli 0111:B4) and FITC-dextran (40,000 Da) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Recombinant mouse GM-CSF and IL-4 were obtained from PeproTech Inc. (RockyHill, NJ, USA). Anti-mouse antibodies FITC-CD11c, -CD40, -CD80, -CD83 and -CD86, Alexa Fluor®647-TLR2 and PE-MHC-II as well as anti-NF-κB p65 were obtained from Biolegend (San Diego, CA, USA). The ELISA kits for IFN-γ, TNF-α, NO, IL-6, IL-10 and IL-12p70 were obtained from eBioscience (San Diego, CA, USA). Antibodies against β-actin, phospho-JNK, JNK, phospho-p38, p38, IκBα, LaminB1 and HRP-conjugated anti-mouse and anti-goat IgG were purchased from SantaCruz Biotech (Santa Cruz, CA, USA), and phospho-ERK1/2 and anti-ERK1/2 antibodies were obtained from BD Pharmingen (San Jose, CA, USA). The inhibitors BAY 11-7082, SP600125, SB203580 and U0126 were purchased from Beyotime Biotechnology (Haimen, Jiangsu, China).