VeroE6 cells (ATCC), VeroCCL81 cells (ATCC), HeLa cells (ATCC) and MDCK cells (gifted from Dr. Andy Vaughan and Dr. Scott Hensley) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% L-glutamine and 4.5g/L D-glucose (Gibco, ThermoFisher) supplemented with 10% heat inactivated fetal bovine serum (FBS) (Hyclone, Cytiva) and 1X penicillin/streptomycin (pen/strep) (Gibco, ThermoFisher). Huh7 cells (ATCC) were grown in the same media supplemented additionally with 1X non-essential amino acids (Gibco). LLC-MK2 cells were cultured in minimal essential media (MEM)-α supplemented with 10% FBS.
4.5 g l d glucose
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
4.5 g/L D-glucose is a laboratory reagent used as a standard solution for various analytical and experimental applications. It provides a known concentration of the monosaccharide D-glucose, which is a common carbohydrate found in many biological samples.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Dexamethasone (dex), by Merck Group (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin pen strep, by Thermo Fisher Scientific (1 mentions)
Hela cell, by American Type Culture Collection (1 mentions)
Huh7 cells, by American Type Culture Collection (1 mentions)
X non essential amino acids, by Thermo Fisher Scientific (1 mentions)
Vero ccl 81 cells, by American Type Culture Collection (1 mentions)
Vero e6 cell, by American Type Culture Collection (1 mentions)
Terbutaline, by Merck Group (1 mentions)
Chlorpromazine, by Merck Group (1 mentions)
Sildenafil, by Merck Group (1 mentions)
Metoprolol, by Merck Group (1 mentions)
Ranitidine, by Merck Group (1 mentions)
Naproxen, by Merck Group (1 mentions)
Cetirizine, by Merck Group (1 mentions)
Hydrochlorothiazide, by Merck Group (1 mentions)
Antipyrine, by Merck Group (1 mentions)
Cimetidine, by Merck Group (1 mentions)
5 protocols using 4.5 g l d glucose
1
Cell Culture Protocol for Virus Studies
2
Culturing Human Glioblastoma Cell Lines
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Human glioblastoma cell lines A172, U87, SNB75 and U251 were obtained from the American Type Culture Collection (ATCC). Cells were cultured in DMEM with 4.5 g/L D-glucose (Gibco, Life Technologies) supplemented with 10% foetal calf serum, 100 units/mL penicillin and 100 μg/ml streptomycin in a humidified incubator at 37°C containing 5% CO2. 2,5-Dimethyl-celecoxib (DMC) was produced as described earlier (Kardosh et al. 2005 (link); Ahlstrom et al. 2007 (link)). Celecoxib (CXB) was purchased from Key Organics. DMC and CXB were both dissolved in DMSO; the control with only solvent (DMSO) showed no toxicity. RhTRAIL wild-type (WT), DR4-specific TRAIL variant 4C7 and DR5-specific TRAIL variant D269H/E195R (amino acids 114–281) were constructed and produced as described earlier (van der Sloot et al. 2006 (link); Reis et al. 2010 (link)).
3
Caco-2 Cell Permeability Assay
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Caco-2 cells of passage number 47 were purchased from Cell Culture Collections, Public Health England (Salisbury, UK). Dulbecco's modified eagle medium (DMEM) supplemented with GlutaMAX™, 4.5 g/L D-glucose and 25 mM 4-2-hydroxyethyl-1piperazineethanesulfonic acid (HEPES) was purchased from Gibco (Paisley, UK). Pravastatin was obtained from Kemprotec Ltd (Lancashire, UK). Hank's balanced salt solution (HBSS), HEPES buffer, fetal bovine serum (FBS), antipyrine, cetirizine, chlorpromazine, cimetidine, desipramine, dexamethasone, diclofenac, furosemide, hydrochlorothiazide, ketoprofen, metoprolol, naproxen, piroxicam, propranolol, ranitidine, sildenafil, terbutaline and verapamil were purchased from Sigma (Gillingham, UK). Corning 24-well Transwell ® was purchased from Fisher Scientific (Loughborough, UK). All solvents were HPLC grade or higher and all other chemicals were analytical reagent grade or higher.
4
Culturing and Differentiating Cell Lines
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COS-7 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) containing GlutaMAX-I, 1 g/L D-glucose, and sodium pyruvate (Life Technologies, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 µg/mL streptomycin (all from PAA, Cölbe, Germany). HepG2, C2C12, and 3T3-L1 cells were cultured in DMEM containing L-glutamine, 4.5 g/L D-glucose, and sodium pyruvate (Life Technologies) supplemented with 10% FBS, 100 U/mL penicillin, and 100 µg/mL streptomycin. C2C12 cells were induced to differentiation upon 80% confluence by serum withdrawal (DMEM with 2% horse serum; PAA) for seven days. 3T3-L1 cells were differentiated as follows: two days after cells reached 80% confluency, adipocyte differentiation was initiated by treatment with growth medium containing 10 μg/mL insulin, 1 μM dexamethasone, and 0.5 mM isobutylmethylxanthine (all from Sigma-Aldrich, Steinheim, Germany) for two days. Cells were then treated with growth medium containing 10 μg/mL insulin for two days. Cells were then maintained in regular growth medium (DMEM/10% FBS) for an additional four days. Differentiation was achieved by day eight. Cell incubation was performed at 37 °C in a humidified 5% CO2 atmosphere.
5
Isolation and Expansion of Human Chondroprogenitor Cells
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Human epiphyseal chondroprogenitor cells were prepared as described elsewhere, 18 and distributed in standard polystyrene tissue culture flasks (75 cm 2 ) for monolayer expansion. Cell culture medium (CCM) was made from Dulbecco's modified Eagle's medium with l-glutamine, 4.5 g/L d-glucose, and sodium pyruvate, (Life Technologies, Paisley, UK), supplemented with 10% fetal bovine serum (Sigma, St. Louis, MO, USA) and 1% l-glutamine (Life Technologies). Culture flasks containing cells were incubated in standard conditions (at 37°C with 5% CO 2 ) and the CCM was changed twice a week in monolayer culture period. After 90% confluence, cells were trypsinized for passage (up to passage 7) or used in seeding experiments.
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