Tear samples were obtained non-traumatically from the inferior tear meniscus. 5 μL of tear was collected with glass capillary micropipettes. Tear samples were immediately eluted fully and then collected into a sterile collection tube. During collection, the tube was kept cold (4°C), and after collection, it was placed at the temperature of −80°C until the activity assays were performed. The concentrations of IL11 in the tears were analyzed using a Human IL-11 ELISA Kit (ELH-IL11, RayBiotech, USA).
Human il 11 elisa kit
Manufactured by RayBiotech
Sourced in United States
The Human IL-11 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-11 (IL-11) levels in biological samples.
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Lab products found in correlation
2 protocols using human il 11 elisa kit
1
Non-invasive Tear Collection and IL-11 Analysis
2
Quantification of Secreted Cytokines via ELISA
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Secreted IL-6, IL-11, LIF, and OSM in the media supernatant of cultured cells was quantified using a LEGEND MAX Human IL-6 ELISA Kit (Biolegend #430507), Human LIF ELISA Kit (Raybiotech #ELH-LIF-1, Peachtree Corners, GA, USA), Human OSM ELISA Kit (Raybiotech #ELH-OSM-1), and Human IL-11 ELISA Kit (Raybiotech #ELH-IL11-1), as per the manufacturer’s instructions. Absorbance at 450 nm was measured on a microplate reader (PE Enspire).
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