Pheochromocytoma cells (PC-12, ATCC® CRL-1721™) were purchased from ATCC. p-Cresol was purchased from UHN Shanghai R&D Co. Ltd. Anti-NF-M/H (RMdO-20) primary antibody (sc-32273), poly-D-lysine (sc-136156), and RIPA lysis buffer system (sc-24948) were purchased from Santa Cruz Biotechnology. Horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody (ab6814) and a human BDNF ELISA Kit (ab99978) were purchased from Abcam plc. Native mouse NGF 2.5S protein (99%) (N-245) was purchased from Alomone Labs. Dulbecco's modified Eagle's medium (DMEM), high glucose, GlutaMAX™ Supplement, pyruvate (#10569010, Gibco), horse serum (HS), fetal bovine serum (FBS), and micro BCA™ Protein Assay Kit (cat.no: 23235) were purchased from ThermoFisher Scientific. Albumin V fraction (Art. No. 8076.3; Roth), rizatriptan (Rizeptam 10 mg; Laboratirios Lesvi, Spain), and enkephalin M (Sigma-Aldrich; M6638) were also procured.
Ripa lysis buffer system sc 24948
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The RIPA lysis buffer system (sc-24948) is a cell lysis solution designed to extract proteins from cells and tissues for analysis. It is a commonly used buffer that provides efficient protein solubilization and denaturation, allowing for the extraction of a wide range of proteins from various sample types.
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2 protocols using ripa lysis buffer system sc 24948
1
Establishing PC-12 Cell Culture Protocol
2
Reagents and Inhibitors for Signaling Pathways
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Chemicals DSS (199-08361) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 1-Hydroxy-2-oxo-3,3-bis-(2-aminoethyl)-1-triazene (NOC18) (N379) was purchased from Dojindo (Kumamoto, Japan). Sodium orthovanadate (198-09752), RIPA Lysis Buffer System (sc-24948), and STAT3 inhibitor S3I-201 (sc-204304) were obtained from Santa Cruz Biotechnology (Dallas, TX, U.S.A.). Immobilon western chemiluminescent horseradish peroxidase substrate (WBKLS0500) and NF-κB activation inhibitor BMS-345541 (401480-1MGCN) were purchased from Merck Millipore Corporation (Darmstadt, Germany). A protease inhibitor cocktail (K271-500) was purchased from BioVision (Milpitas, CA, U.S.A.). TLR4 inhibitor VIPER (NBP2-26244) and LPS from Escherichia coli serotype O55:B5 (L2880-25MG) were obtained from Novus Biologicals (Littleton, CO, U.S.A.) and Sigma-Aldrich (St. Louis, MO, U.S.A.), respectively. All other reagents were purchased from Wako Pure Chemical Industries, Ltd.
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