Rat hearts lysates (500 mg protein) were immunoprecipitated for SERCA2 using monoclonal mouse antibody (2 μg/ml, Thermo Scientific) with 20 μl protein G magnetic beads (Cell Signaling Technology). Immunoprecipitated protein captured on the beads was eluted in the sample buffer and resolved on polyacrylamide gels for subsequent Western blotting using SERCA and Br-Tyr antibody as described previously (Ahmad et al. 2019 (link)). Immunoblots on cardiac tissues were performed according to previously described methods using antibodies against SERCA2 (1:1000 abcam), Br-Tyrosine (1:1000; JaICA), phospho-phospholamban (1:1000, Cell Signaling Technology), phospholamban (1:1000, Cell Signaling Technology), PP1 (1:1000 R&D Systems), NOX2 & NOX4 (1:500 Novus Biologicals), GAPDH (1:5000, Cell Signaling Technology), and 4-Hydroxynonenal (1:1000 abcam).
Monoclonal mouse antibody
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Monoclonal mouse antibody is a laboratory reagent that is produced from a single clone of mouse B cells. It binds specifically to a target antigen and can be used for various applications in research and diagnostic settings.
Automatically generated - may contain errors
Lab products found in correlation
4 hydroxynonenal, by Abcam (1 mentions)
Phospholamban, by Cell Signaling Technology (1 mentions)
Protein g magnetic beads, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Monoclonal mouse antibody, by Santa Cruz Biotechnology (1 mentions)
Ecl prime kit, by Cytiva (1 mentions)
Secondary anti mouse hrp antibody, by Santa Cruz Biotechnology (1 mentions)
M per lysis buffer, by Thermo Fisher Scientific (1 mentions)
2 protocols using monoclonal mouse antibody
1
SERCA2 Immunoprecipitation and Oxidative Modifications
2
Measuring HMG-CoA Reductase in Fluvastatin-Resistant Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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WT and fluvastatin-resistant replicon containing cells were cultured in the absence or presence of 5 µM fluvastatin for 24h. Cells were lysed in M-PER lysis buffer (ThermoScientific, Erembodegem, Belgium), denatured and loaded onto a 4-20% SDS-PAGE gel. After separation and transfer to a PVDF membrane, immunoblotting was performed to detect HMG-CoA reductase using a monoclonal mouse antibody
(1:100, Santa Cruz, Heidelberg, Germany) and β-actin as endogenous control with a monoclonal mouse antibody (1:10000, ThermoScientific, Erembodegem, Belgium).
The secondary anti-mouse HRP antibody (Santa Cruz, Heidelberg, Germany) was used at a dilution of 1:1000. Bound antibodies were visualized using the ECL Prime kit (Amersham, Diegem, Belgium) and the BioRad imaging system.
(1:100, Santa Cruz, Heidelberg, Germany) and β-actin as endogenous control with a monoclonal mouse antibody (1:10000, ThermoScientific, Erembodegem, Belgium).
The secondary anti-mouse HRP antibody (Santa Cruz, Heidelberg, Germany) was used at a dilution of 1:1000. Bound antibodies were visualized using the ECL Prime kit (Amersham, Diegem, Belgium) and the BioRad imaging system.
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