Cells were washed twice in ice-cold PBS and then lysed in DET buffer (150 mM NaCl, 25 mM Hepes pH 7.5, 1 mM β-Mercaptoethanol, 0.2 mM CaCl 2 , 0.5 mM MgCl 2 , 0.5% NP-40) supplemented with PhosStop and Complete Ultra Protease Inhibitor tablets (Roche, Welwyn Garden City, UK). Lysates were incubated on ice for 10 minutes and centrifuged. The resulting pellets were washed twice in DET buffer, resuspended in RIPA buffer and cleared by centrifugation. For mass spectrometry, nuclear lysates were prepared using Nuclei PURE prep isolation kit (Sigma, Gillingham, UK).
Phosstop and complete ultra protease inhibitor tablets
Manufactured by Roche
Sourced in United Kingdom
PhosStop and Complete Ultra Protease Inhibitor tablets are laboratory products manufactured by Roche. PhosStop is a phosphatase inhibitor, while Complete Ultra Protease Inhibitor tablets are used to inhibit a wide range of proteases. Both products are intended for use in scientific research and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phosstop and complete ultra protease inhibitor tablets
1
Nuclear Protein Extraction and Purification
2
Cell Lysis and Protein Extraction
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Cells were washed twice in ice-cold PBS and then lysed in RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% SDS and 0.5% sodium deoxycholate) supplemented with PhosStop and Complete Ultra Protease Inhibitor tablets (Roche, Welwyn Garden City, UK) and cleared by centrifugation.
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