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Mouse anti ha ha 11

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Mouse anti-HA (HA.11) is a monoclonal antibody that recognizes the influenza hemagglutinin (HA) epitope tag. It is commonly used for the detection and immunoprecipitation of HA-tagged proteins in various applications.

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Lab products found in correlation

Mouse anti flag m2, by Merck Group (5 mentions) Rat anti ha 3f10, by Roche (2 mentions) Mouse anti gfp, by Roche (2 mentions) Gsk 3β, by Abcam (1 mentions) Phospho gsk 3β s9, by Abcam (1 mentions) Anti rabbit igg alexa fluor plus 488, by Thermo Fisher Scientific (1 mentions) Anti mouse igg alexa fluor plus 594, by Thermo Fisher Scientific (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Alexa 633, by Thermo Fisher Scientific (1 mentions) Tcs sp5 confocal microscope, by Leica (1 mentions) Rabbit polyclonal anti lamin b1, by Abcam (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Mouse anti β tubulin, by Merck Group (1 mentions) Rabbit anti histone h3, by Abcam (1 mentions) Rabbit anti stat2, by Santa Cruz Biotechnology (1 mentions) Tritc conjugated phalloidin, by Merck Group (1 mentions) Horseradish peroxidase conjugated anti mouse and anti rabbit igg, by GE Healthcare (1 mentions) Mowiol, by Merck Group (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Image studio 5, by LI COR (1 mentions)

Close spelling variants of this product

Anti-HA (161 citations) Mouse anti-HA (96 citations) HA.11 (55 citations) Anti-HA.11 (19 citations) Mouse anti-HA.11 (8 citations) Mouse anti-HA.11 clone 16B12 (3 citations) Mouse anti-HA antibody (HA.11, (2 citations) Mouse anti-HA mAb (HA.11, (2 citations) Mouse monoclonal anti-HA (HA.11 clone 16B12, (2 citations) Mouse anti-HA HA.11 clone 16B12 (2 citations)

8 protocols using mouse anti ha ha 11

1

Investigating Endocytic Trafficking Pathways

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All reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated. The following antibodies were purchased from Cell Signal Technologies (Danvers, MA, USA): phospho-AKT S473 (#9271), AKT (#9272), phospho-mTOR S2481 (#2974), mTOR (#2972), LAMP1 (Clone D2D11, #9091), EEA1 (#2411), Rab7 (Clone D95F2, #9367), Rab11 (Clone D4F5, #5589), β-actin (Clone 13E5, #4970), anti-rabbit IgG HRP (#7074), and anti-mouse IgG HRP (#7076). The following antibodies were purchased from Abcam (Cambridge, UK): phospho-GSK-3β S9 (ab131097), GSK-3β (Clone 3D10, ab93926), and megalin (ab76969). Mouse anti-HA (HA.11) was purchased from Covance (Princeton, NJ, USA). The following fluorescent antibodies were obtained from Thermo Fisher Scientific (Waltham, MA, USA): anti-rabbit IgG Alexa Fluor Plus 488 (A32731), anti-rabbit IgG Alexa Fluor Plus 594 (A32754), anti-mouse IgG Alexa Fluor Plus 488 (A32723), and anti-mouse IgG Alexa Fluor Plus 594 (A32744). Proteinuria and urinary creatinine colorimetric kits were purchased from Labtest (MG, Brazil). Wortmannin (CAS 19545-26-7) was purchased from Calbiochem (San Diego, CA, USA). MK-2206 (S1078) was purchased from Selleckchem (Houston, TX, USA). Albumin-fluorescein isothiocyanate (FITC) (A9771) was purchased from Sigma-Aldrich. DQ-Albumin Green (D12050) and shRNA for megalin (Ambion) were purchased from Thermo Fisher Scientific.
Silva-Aguiar R.P., Peruchetti D.B., Florentino L.S., Takiya C.M., Marzolo M.P., Dias W.B., Pinheiro A.A, & Caruso-Neves C. (2022). Albumin Expands Albumin Reabsorption Capacity in Proximal Tubule Epithelial Cells through a Positive Feedback Loop between AKT and Megalin. International Journal of Molecular Sciences, 23(2), 848.
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2

Nef-TagRFP657 Localization Assay

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JTAg cells were electroporated with constructs expressing Nef-TagRFP657 or the control TagRFP657, Nef–HA, HA–glycoMa, SERINC5–GFP and Rab7–RFP as indicated. Then 48 h after transfection, cells were overlaid on poly-l-lysine coated glass slides, fixed with 4% paraformaldehyde and permeabilzed with 0.1% Triton X-100. The HA tag was detected by staining with mouse anti-HA (HA.11, Covance) and the secondary antibody Alexa 633 (Life Technologies). Images were acquired using a Leica TCS SP5 confocal microscope.
Rosa A., Chande A., Ziglio S., Sanctis V.D., Bertorelli R., Goh S.L., McCauley S.M., Nowosielska A., Antonarakis S.E., Luban J., Santoni F.A, & Pizzato M. (2015). HIV-1 Nef promotes infection by excluding SERINC5 from virion incorporation. Nature, 526(7572), 212-217.
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3

Antibodies for Immunofluorescence and Analysis

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The following antibodies were used for immunofluorescence, FACS and immunoblot analysis: goat anti-Salmonella (CSA-1, Kirkegaard and Perry Laboratories), rabbit anti-DnaK [63 (link)], rat anti-HA (3F10, Roche), mouse anti-HA (HA11, Covance), mouse anti-FLAG (M2, Sigma-aldrich), rabbit anti-myc (Cell Signaling), mouse anti-β tubulin (Sigma-aldrich), rabbit polyclonal anti-GAPDH (Abcam), rabbit or goat anti-p65 (Santa Cruz), mouse anti- IĸBα (Cell Signaling), rabbit polyclonal anti-lamin B1 (Abcam), rabbit polyclonal anti-Xpo2 (CSE1L, Abcam), rabbit anti-Xpo1 (CRM1, Santa Cruz), rabbit anti-Xpo5 (Sigma-aldrich), rabbit anti-histone H3 (Abcam), rabbit anti-KPNA1 (Proteintech), mouse anti-p50 (Biolegend) and rabbit anti-STAT2 (Santa Cruz). Alexa Fluor 488-, 555- and 633- conjugated donkey anti-rat, anti-mouse, anti-goat and anti-rabbit antibodies were from Life technologies, UK.
Rolhion N., Furniss R.C., Grabe G., Ryan A., Liu M., Matthews S.A, & Holden D.W. (2016). Inhibition of Nuclear Transport of NF-ĸB p65 by the Salmonella Type III Secretion System Effector SpvD. PLoS Pathogens, 12(5), e1005653.
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4

Antibody Characterization and Immunoblotting

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The primary antibodies used were mouse anti-HA HA11 (Covance), mouse anti-GFP (Roche Applied Science), mouse anti-FLAG M2 (Sigma), mouse anti-α-tubulin (Sigma), and mouse anti-epidermal growth factor receptor (Santa Cruz Biotechnology). Rabbit anti-EFA6R antibody was produced against a peptide representing the last 15 C-terminal amino acids of EFA6R and affinity-purified by using the immunizing peptide-coupled resin through a commercial vendor (Eurogentec). Cy3- and Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories) secondary antibodies and TRITC-conjugated phalloidin (Sigma) were used for immunofluorescence. Horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG (GE Healthcare) secondary antibodies were used for immunoblotting. INSTA-Blot human tissues and Mowiol were from Merck Millipore. All other chemicals were from Sigma unless otherwise specified.
, & Kanamarlapudi V. (2014). Exchange Factor EFA6R Requires C-terminal Targeting to the Plasma Membrane to Promote Cytoskeletal Rearrangement through the Activation of ADP-ribosylation Factor 6 (ARF6). The Journal of Biological Chemistry, 289(48), 33378-33390.
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5

Quantitative Western Blot Protocol

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For western immunoblotting, samples were fixed in TCA, acetone-washed and whole cell extracts prepared by bead-beating in TE containing protease inhibitors before running on SDS-PAGE and transferring to nitrocellulose membrane. Antibodies used were mouse anti-Ha (HA11, Covance) at 1:1000 dilution, mouse anti-GFP (Roche) at 1:1000, mouse anti-V5 (SV5-Pk1, Bio-Rad) at 1:2000, mouse anti-Flag (M2, Sigma) at 1:1000, rabbit anti-Pgk1 (lab stock) at 1:10000, rabbit anti-Kar2 (lab stock) at 1:20000, sheep anti-mouse-HRP (GE Healthcare) at 1:5000, donkey anti-rabbit-HRP (GE Healthcare) at 1:10000, donkey anti-mouse-IRDye 800CW (LI-COR Biosciences) at 1:10000 and donkey anti-rabbit-IRDye 680RD (LI-COR Biosciences) at 1:10000. Quantitative western blotting was performed using an Odyssey CLx Infrared Imaging System (LI-COR Biosciences) and quantified using ImageStudio 5.2.5 (LI-COR Biosciences).
Galander S., Barton R.E., Borek W.E., Spanos C., Kelly D.A., Robertson D., Rappsilber J, & Marston A.L. (2019). Reductional Meiosis I Chromosome Segregation Is Established by Coordination of Key Meiotic Kinases. Developmental Cell, 49(4), 526-541.e5.
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6

Antibody Immunofluorescence and ChIP Assays

Cited 1 time
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The following antibodies were used for immunofluorescence assays: mouse anti-GFP (LGB-1; Abcam), rabbit anti-RFP (ab62341; Abcam); mouse anti-Fibrillarin (38F3; Abcam), mouse anti-HA (HA.11; Covance) and rabbit anti-Rep1 (custom made; directed to a synthetic Rep1 peptide) (31 (link)). A mouse anti-Myc antibody (9E10; Covance) was used in the chromatin immunoprecipitation (ChIP) assays. Antibody dilutions ranged from 1:100 to 1:500 in individual assays, in accordance with initial standardization.
Liu Y.T., Chang K.M., Ma C.H, & Jayaram M. (2016). Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome. Nucleic Acids Research, 44(17), 8302-8323.
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7

Antibodies for Hedgehog Pathway Research

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Anti-Su(fu) (25H3) and anti-Fu (26F11) mouse monoclonal antibodies (mAbs) and anti-Cos2 rabbit polyclonal antiserum have been previously described [46 (link)]. Other antibodies used are: anti-Ci (2A1) rat mAb (a gift from R. Holmgren), rabbit anti-Lamin Dm0 (R836) (a gift from P. Fisher), anti-β-Tubulin mAb (E7) (Developmental Studies Hybridoma Bank), rat anti-HA (3F10) (Roche), mouse anti-HA (HA11) (Covance), mouse anti-V5 (Invitrogen), mouse anti-Myc (9E10), rabbit anti-Myc (A14) and rabbit anti-Sufu (Santa Cruz Biotechnology), mouse anti-FLAG (M2), mouse anti-acetylated α-Tubulin (Sigma), mouse anti-β-galactosidase (Promega), rabbit anti-GFP (Invitrogen), mouse anti-Nkx2.2, Foxa2 and Isl1/2 (Developmental Studies Hybridoma Bank), rabbit anti-Pax6 (Covance), guinea pig anti-Gli2 (a gift from J. Eggenschwiler), and rabbit anti-Gli2 (a gift from B. Wang).
Oh S., Kato M., Zhang C., Guo Y, & Beachy P.A. (2015). A Comparison of Ci/Gli Activity as Regulated by Sufu in Drosophila and Mammalian Hedgehog Response. PLoS ONE, 10(8), e0135804.
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8

Immunofluorescence and Immunoblotting Protocols

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The following primary antibodies were used for immunofluorescence labeling or immunoblotting: mouse anti-ACBD3 (Sigma) at a 1:1,000 dilution for immunofluorescence labeling and a 1:3,000 dilution for immunoblotting; mouse anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) (Abcam) at a 1:1,000 dilution and mouse anti-Flag (M2; Sigma) at a 1:2,000 dilution for immunoblotting; rabbit antigiantin (Covance) at a 1:1,000 dilution, rabbit anti-TGN46 (LifeSpan Biosciences) at a 1:500 dilution, and goat anti-Salmonella antibody (CSA-1; Kirkegaard & Perry Laboratories) at a 1:200 dilution for immunofluorescence labeling; and mouse anti-HA (HA.11; Covance) at a 1:5,000 dilution for immunoblotting and a 1:1,000 dilution for immunofluorescence labeling.
Rhodamine Red X-conjugated donkey anti-mouse or anti-rabbit antibody, donkey anti-goat or donkey anti-rabbit–cyanine 2 (Cy2) antibody, and Cy5-conjugated donkey anti-mouse antibody were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA), for immunofluorescence labeling. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse antibodies were purchased from Dako for immunoblotting.
Yu X.J., Liu M, & Holden D.W. (2016). Salmonella Effectors SseF and SseG Interact with Mammalian Protein ACBD3 (GCP60) To Anchor Salmonella-Containing Vacuoles at the Golgi Network. mBio, 7(4), e00474-16.
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