Tlc silica gel 60f254 plates

All reactions were performed in oven-dried glassware under an argon atmosphere unless otherwise stated. Commercially available anhydrous solvents and reagents were utilized during synthesis. Flash column chromatography was performed using silica gel (40–63 μm particle size). The 1H spectra were recorded on a Brucker instrument at 500, and 400 MHz frequencies and 13 C NMR were recorded at 126, and 101 MHz frequencies. Data are reported as p=pentet, q=quartet, t=triplet, d=doublet, s=singlet, bs=broad singlet, m=multiplet, coupling constant(s) in Hz. High resolution mass spectral data were obtained on a time-of-flight mass spectrometer, and analysis was performed using electrospray ionization. The final products were determined to be ≥ 95% purity using an Agilent technologies Infinity II instrument with a Poroshell 120 EC-C18 2.7 μm (4.6×100 mm) column using a solvent gradient of solvent A (H2O with 0.1% trifluoroacetic acid) and solvent B (acetonitrile with 0.1% trifluoroacetic acid). Thin-layer chromatography was performed with TLC silica gel 60F254 plates purchased from Millipore Sigma and visualized by UV light.

All reactions were performed in oven-dried glassware under argon atmosphere unless otherwise stated. Commercially available anhydrous solvents and reagents were utilized during synthesis. Flash column chromatography was performed using silica gel (40−63 μm particle size). The 1H spectra were recorded on Brucker instrument at 600, 500 and 400 MHz frequency and 13C NMR were recorded at 151,126 and 101 MHz frequencies. Data are reported as p = pentet, q = quartet, t = triplet, d = doublet, s = singlet, bs = broad singlet, m = multiplet; coupling constant(s) in Hz. High resolution mass spectral data were obtained on a time-of-flight mass spectrometer and analysis was performed using electrospray ionization. The final products were determined to be > 95% purity using Agilent technologies Infinity II instrument with a Poroshell 120 EC-C18 2.7 μm (4.6 X 100 mm) column using a solvent gradient of Solvent A (H2O with 0.1 % Trifluoroacetic acid) and Solvent B (Acetonitrile with 0.1% Trifluoroacetic acid ). Thin-layer chromatography was performed with TLC Silica gel 60 F254 plates purchased from Millipore Sigma and visualized by UV light.