PAC assay was accomplished using direct ELISA assays (DiaMetra Ekit, Spello, Italy) with a reference range in the upright position of 3–40 ng/dL and in the supine position of 2–18 ng/dL. The intra-assay variability was < 9.7%, and the inter-assay variability was < 11%. PRA was determined using a direct ELISA assay (DRG Instruments GmBH, Germany) with a reference range of 0.06–4.69 ng/mL/hr. Serum cortisol levels were measured by electrochemiluminescence assay (ECLIA) (Elecsys®, Cortisol II assay, Roche Diagnostics GmbH, Mannheim, Germany).
Direct elisa assay
Manufactured by DRG International
Sourced in Germany
The Direct ELISA assay is a laboratory technique used to detect and quantify specific proteins or antigens in a sample. It is a type of enzyme-linked immunosorbent assay (ELISA) where the target analyte is directly captured on a solid surface and detected using an enzyme-labeled antibody. The core function of the Direct ELISA assay is to provide a sensitive and specific method for the measurement of target analytes in various sample types.
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Lab products found in correlation
2 protocols using direct elisa assay
1
Adrenal Hormone Assay Protocol
2
Measurement of Hormonal Biomarkers
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PAC was measured using direct ELISA assays (DiaMetra Ekit, Spello, Italy) with a reference range in the upright position of 3–40 ng/dL and in the supine position of 2–18 ng/dL. The intra-assay variability was < 9.7% and the inter-assay variability was < 11%. PRA was determined using direct ELISA assay (DRG Instruments GmBH, Germany) with a reference range of 0.06–4.69 ng/mL/hr. For assessment of insulin, electrochemiluminescence immunoassay (Electrosys Insulin, Cobas, Roche Diagnostics, Switzerland) was employed with reference values of 2.6–24.9 μU/mL and with intra- and inter-assay coefficients of variation for serum insulin of < 10%.
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