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1 2 dilauroyl sn glycero 3 phosphocholine

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1,2-dilauroyl-sn-glycero-3-phosphocholine is a synthetic phospholipid compound. It serves as a structural component for the formation of lipid bilayers and liposomes.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (8 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (3 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (3 mentions) Polyethylene glycol, by Merck Group (2 mentions) 3 3 dioctadecyloxacarbocyanine, by Merck Group (2 mentions) 1 2 dilauroyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphoglycerol, by Avanti Polar Lipids (1 mentions) 1 heptadecanoyl 2hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Takara bca protein assay kit, by Takara Bio (1 mentions) Potassium chloride (kcl), by Fujifilm (1 mentions) Hepes, by Dojindo Laboratories (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (1 mentions) Octadecyltrichlorosilane, by Thermo Fisher Scientific (1 mentions) α hemolysin, by Merck Group (1 mentions) Squalene, by Thermo Fisher Scientific (1 mentions) N hexadecane, by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Nahco3, by Thermo Fisher Scientific (1 mentions) Alamethicin, by Merck Group (1 mentions)

15 protocols using 1 2 dilauroyl sn glycero 3 phosphocholine

1

Preparation of Lipid Vesicles

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1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE), 1,2-dilauroyl-sn-glycero-3-phosphoglycerol (DLPG), 1,2dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), 1,2-dipalmitoyl-sn-glycero-3-phosphophosphoglycerol (DPPG), 1,2-distearoyl-sn-glycero-3-phosphophosphoglycerol (DSPG) and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) were purchased from Avanti Polar Lipids Inc. The lipids were dissolved in chloroform (DLPE) and chloroform:methanol 5:1 (other lipids) separately, then mixed together to achieve desired stoichiometry. Total lipid concentration was 30 mg/ml per sample. The solution was evaporated overnight in a fume hood, and re-fluidized using a buffer at 6.7 pH containing 20 mM 2-(N-Morpholino)ethanesulfonic acid (MES), 1 mM MgCl2 and 13 mM NaOH. NaCl was added to retain desired monovalent salt concentration. Samples were then placed in an incubator at 37 • C for 3 hours, and homogenized using a vortexer every 25 minutes. Samples were then placed in quartz capillaries, containing about 100 µl, and centrifuged for 5 minutes at 3000 rpm, to create a pellet of lipids.
Jacoby G., Portnaya I., Danino D., Diamant H, & Beck R. (2020). Delayed nucleation in lipid particles. Soft matter, 16(1).
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2

Lipid Extraction from Exosomes

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Lipids the exosomes were extracted using the Bligh and Dyer technique. 27 The chloroform-methanolaqueous (1:2:0.8, v/v/v) mixture was stirred at 4 °C for 30 min. Then, 10 mmol/L of Tris-HCl (pH 7.4) and chloroform were added. The mixtures were centrifuged for 15 min at 1000 × g. For internal standards, 1,2dilauroyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Alabaster, AL, USA) and 1-heptadecanoyl-2hydroxy-sn-glycero-3-phosphocholine and were added. The upper and the lower phases were collected using chloroform and nitrogen gas stream respectively. Lipids obtained by the above process were dissolved with chloroform-methanol (2:1, v/v) and stored at -20 °C.
Senda A., Kojima M., Watanabe A., Kobayashi T., Nakatsutsumi K., Yagi M., Morishita K., Aiboshi J., & Otomo Y. (2021). Investigating Closed Room Conspirators of Posttraumatic Inflammatory Response: Multi- Omics Profiles of Exosomes Derived from Intestinal Epithelial Cells Under Ischemia Reperfusion Injury.
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3

In Vitro Reconstitution of Membrane Proteins

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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (DOPG), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), and Escherichia coli (E. coli) extract polar were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). PUREfrex2.0 was purchased from Cosmo Bio (Tokyo, Japan). A pre-cast gel of 15% SDS-polyacryamide gel was purchased from ATTO (Tokyo, Japan). HEPES was purchased from DOJINDO LABORATORIES (Kumamoto, Japan). KCl and sucrose were purchased from FUJIFILM Wako Chemicals (Tokyo, Japan). TaKaRa BCA protein assay kit was purchased from TaKaRa BIO (Shiga, Japan).
, & Kamiya K. (2022). Formation and function of OmpG or OmpA-incorporated liposomes using an in vitro translation system. Scientific Reports, 12, 2376.
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4

Lipid Bilayer Formation Reagents

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The phospholipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoglycerol (DOPG), 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC), and 1,2dilauroyl-sn-glycero-3-phosphocholine (DLPC) were obtained from Avanti Polar Lipids. Squalene, n-hexadecane, and octadecyltrichlorosilane (OTS) were purchased from Acros Organics. NaCl (99.99%, metals basis) and NaHCO 3 were obtained from Alfa Aesar. CaCl 2 , α-hemolysin, and alamethicin was purchased from Sigma-Aldrich. Ultra-pure water (Milli-Q, Merck-Millipore, resistivity < 18.2 MΩ•cm) was used to make all buffers.
Beltramo P.J., Scheidegger L, & Vermant J. (2018). Toward Realistic Large-Area Cell Membrane Mimics: Excluding Oil, Controlling Composition, and Including Ion Channels. Langmuir : the ACS journal of surfaces and colloids, 34(20).
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5

Curcumin-Loaded Nanoparticle Synthesis

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Curcumin (≧ 94% purity), polyethylene glycol (PEG, MW 1500 and 8000), polyethyleneimine (branched PEI, MW 25,000) and 3,3′-dioctadecyloxacarbocyanine (DiO) were purchased from Sigma-Aldrich (St. Louis, MO). 1,2-Dioleoyl-sn-Glycero-3-Phosphocholine (DOPC) and 1,2-Dilauroyl-sn-Glycero-3-Phosphocholine (DLPC) were purchased from Avanti Polar Lipids (Alabaster, AL). Herceptin was purchased from Roche (Basel, Switzerland). Rabbit polyclonal antibody to human IgG F(c)-FITC was purchased from Acris Antibodies GmbH (Herford, Germany).
Lin Y.L., Tsai N.M., Chen C.H., Liu Y.K., Lee C.J., Chan Y.L., Wang Y.S., Chang Y.C., Lin C.H., Huang T.H., Wang C.C., Chi K.H, & Liao K.W. (2019). Specific drug delivery efficiently induced human breast tumor regression using a lipoplex by non-covalent association with anti-tumor antibodies. Journal of Nanobiotechnology, 17, 25.
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6

Lipid and Fluorescent Dye Characterization

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Sphingomyelin (SM) from porcine brain and chicken egg, 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were from Avanti Polar Lipids. 8-Aminonaphthalene-1,3,6-trisulfonic acid (ANTS), p-xylene-bis-pyridinium bromide, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC18), and Alexa Fluor 633 succinimidyl ester were from Thermo Fisher Scientific. Proteinase K (PK) was purchased from Sigma.
Morante K., Bellomio A., Gil-Cartón D., Redondo-Morata L., Sot J., Scheuring S., Valle M., González-Mañas J.M., Tsumoto K, & Caaveiro J.M. (2016). Identification of a Membrane-bound Prepore Species Clarifies the Lytic Mechanism of Actinoporins. The Journal of Biological Chemistry, 291(37), 19210-19219.
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7

Phospholipid Purchase and Storage

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1,2-dipalmitoyl-sn-glycero-3-phosphocholine (hDPPC), 1,2-dipalmitoyl-d62-sn-glycero-3-phosphocholine (dDPPC) and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA) as lyophilized powders and stored at −80 °C until further use.
Krzyzanowski N., Porcar L, & Perez-Salas U. (2023). A Small-Angle Neutron Scattering, Calorimetry and Densitometry Study to Detect Phase Boundaries and Nanoscale Domain Structure in a Binary Lipid Mixture. Membranes, 13(3), 323.
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8

Lipid Synthesis and Characterization

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1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). PEG (molecular weight [MW] 1,500 and 8,000), PEI branched, (MW 25,000), p-nitrophenyl-β-d-glucuronide (PNPG), 5-bromo-4-chloro-3-indolyl-β-d-glucuronide cyclohexylamine salt (X-gluc), FITC, and paraformalde-hyde, and 3,3′-dioctadecyloxacarbocyanine, p-nitrophenyl-β-d-glucuronide were obtained from Sigma-Aldrich (St Louis, MO, USA). BSA and trypan blue staining were purchased from Invitrogen (Gaithersburg, MD, USA). Triton X-100 was purchased from Amresco (Solon, OH, USA).
Lin Y.L., Chen C.H., Liu Y.K., Huang T.H., Tsai N.M., Tzou S.C, & Liao K.W. (2019). Lipo-PEG-PEI complex as an intracellular transporter for protein therapeutics. International Journal of Nanomedicine, 14, 1119-1130.
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9

Lipid-based Nanoparticle Synthesis Protocol

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Tetraethylorthosilicate (TEOS) was purchased from Acros Organics. Dodecyltrichlorosilane was purchased from Gelest, Inc. Cetyltrimethylammonium chloride (CTAC, 25 wt.% in water) was purchased from Sigma. Water was deionized with a Milli-Q Advantage A10 system (MilliporeSigma, Inc.) and was used without degassing. Chloroform and hexanes were purchased from Merck. Concentrated hydrochloric acid was obtained from Fischer Scientific. Bovine plasma was ordered from Lampire Biological Laboratories. 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dibehenoyl-sn-glycero-3-phosphocholine (DBPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), were all purchased from Avanti Polar Lipids, Inc. 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[methoxy(polyethylene glycol)- 2000] (DSPE-PEG2k) was also ordered from Avanti Polar Lipids, Inc.
Blum N.T., Yildirim A., Gyorkos C., Shi D., Cai A., Chattaraj R, & Goodwin A.P. (2019). Temperature-Responsive Hydrophobic Silica Nanoparticle Ultrasound Contrast Agents Directed by Phospholipid Phase Behavior. ACS applied materials & interfaces, 11(17), 15233-15240.
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10

Phospholipid-Sterol Monolayer Preparation

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1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), 1,2-didecanoyl-sn-glycero-3-phosphocholine (DDPC), dihydrocholesterol (DChol), and 27-hydroxycholesterol (27OH) were purchased from Avanti Polar Lipids (Alabaster, AL) and used as received. Texas Red DHPE was purchased from Life Technologies (Carlsbad, CA). Phospholipid/sterol ratio mixtures (70/30) were prepared in chloroform from stock solutions with 0.5 mol % Texas Red DHPE to provide contrast between the coexisting phases and those stored at −22 °C. A spreading solution concentration of 0.5 mg/mL was used for all experiments. The 70/30 phospholipid/sterol ratio is approximately the miscibility critical composition.12 Finally, to minimize any effects on monolayer oxidation, we replaced cholesterol with dihydro-cholesterol, and all images and isotherms were taken within 1 h of monolayer deposition to reduce any photo-oxidative effects.35 (link),36
Stottrup B.L., TigreLazo J., Bagonza V.B., Kunz J.C, & Zasadzinski J.A. (2019). Comparison of Line Tension Measurement Methods for Lipid Monolayers at Liquid−Liquid Coexistence. Langmuir : the ACS journal of surfaces and colloids, 35(48), 16053-16061.
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