3h adenosine

EIDD-1931, EIDD2801, and Nirmatrelvir (PF-073213332) were purchased from MedChemExpress (Shanghai, China). Stock solutions of the compounds were freshly prepared in dimethyl sulfoxide (DMSO). Non-radiolabeled chemicals were obtained from Merck/Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade. ³H-Uridine ([5-³H], 20.9 Ci/mmol), and ³H-Adenosine ([2,8-³H], 29.3 Ci/mmol) were purchased from Moravek Biochemicals Inc. (Brea, CA, USA). Ultima Gold XR scintillation fluid was purchased from PerkinElmer (Waltham, MA, USA).

[³H]Adenine (25.0 Ci/mmol), [³H]hypoxanthine (27.0 Ci/mmol), [³H]thymidine (20.0 Ci/mmol), [³H]uridine (14.7 Ci/mmol), [³H]uracil (42.8 Ci/mmol), [³H]xanthine (12.8 Ci/mmol) and [³H]adenosine (39.2 Ci/mmol) were obtained from Moravek Biochemicals (Brea, CA), [¹⁴C]guanine (55 mCi/mmol) was from American Radiolabeled Chemicals (St. Louis, MO), and [¹⁴C]ascorbate (8.5 mCi/mmol) was from PerkinElmer Life Sciences (Boston, MA). Adenine, papaverine, and dipyridamole were obtained from Sigma-Aldrich (St. Louis, MO), and hypoxanthine, guanine and nitrobenzylthioinosine were from Wako Pure Chemicals (Osaka, Japan). All other reagents were of analytical grade and commercially obtained.