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2 protocols using carrageenan

1

Purifying Proteins and Nucleic Acids

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Nucleic acid purification kits were purchased from Huashun Biological Engineering Co., Ltd., Shanghai, China. Restriction enzymes, PCR Ladder Marker, Taq DNA polymerase, pfu Taq, Buffer, and dNTPs were purchased from Nanjing Genescript Co.Ltd. Trizol kits were purchased from Invitrogen. Chelating Sepharose Fast Flow, SP Sepharose Fast Flow were purchased from GE Life Sciences. Indomethacin, carrageenan and xylene were purchased from Solarbio. Plasmid pSYPU-1b, E. coli strain DH5α, E. coli strain BL21, PVDF membrane (Merk Millipore, Billerica, MA), anti-Nav1.7 (1:500, Abcam), anti-Nav1.8 (1:500, Abcam), anti-β-actin (1:2000, Abcam), ELISA kits were purchased from Baolai Biotechnology (Jiangsu, China).
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2

Chicken Breast Protein Extraction

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Distinct chicken breasts (∼880 g) were purchased from Suguo Supermarket (Nanjing, Jiangsu). MOI (≥98%, yellow powder), carrageenan (≥99%, white or yellow powder), locust-bean gum (≥99%, white to light brown powder), and ascorbic acid (≥99%, white powder) were provided by Solarbio Technology Co., Ltd. (Beijing, China). Ethanol (75%, w/v), quaternary ammonium chitosan (90% degree of substitution, white powder), sodium chloride, and other reagents (analytical grade) were supplied by Sigma Aldrich Trading Co., Ltd. (Shanghai, China).
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