One-month-old Acvr1cR206H/+ mice were provided doxycycline chow (Harlan Laboratories, Madison, WI) for 3 days to induce mutant gene expression globally. Mouse quadriceps muscles were injured by injection with 50 μl of 10 μM cardiotoxin (Sigma). Beginning on the day of injury, Palovarotene or vehicle (1:4 DMSO in corn oil) was administered daily for 14 days by oral gavage (100μg/mouse from day 1–3 and 15μg/mouse from day 4–14) using a 20-gauge gavage needle (Fine Science Tools). Palovarotene (R667, Atomax Chemicals, China) solution in DMSO was stored at −20°C under argon and diluted (1:4) with corn oil for administration.
20 gauge gavage needle
Manufactured by Fine Science Tools
Sourced in United States
The 20-gauge gavage needle is a laboratory equipment item used for administering substances directly into the stomach or esophagus of small animals. It has a stainless steel construction and a blunt tip designed for precise and controlled delivery.
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4 protocols using 20 gauge gavage needle
1
Palovarotene Enhances Muscle Regeneration
2
Intragastric Administration of BBS
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Intragastric (i.g.) administrations of BBS were performed as described previously29 (link). Briefly, 100 µL of BBS was administered via a 20-gauge gavage needle (Fine Science Tools Inc., Foster City, California, USA) on Days -21, -14 and -7.
3
Retinoid Agonists and Antagonists Synthesis
4
Microbiome Transplantation in Germ-free Mice
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Germ-free wild-type (WT) and IL-10-deficient (Il-10−/−) mice on 129Sv/Ev background were kept at the gnotobiology core facility of the Institute for food and health, Technical University Munich, Germany. Germ-free mice were housed in flexible film isolators ventilated via HEPA-filtered air at 22 ± 1 °C with a 12-h light/dark cycle. Before experiments, littermates were combined and randomly assigned to treatment groups. A maximum of five mice are housed per cage (floor area ~540 cm2). Mice received a standard diet (autoclaved V1124-300; Ssniff, Soest, Germany) and autoclaved water ad libitum.
For fecal microbiota transplantation, GF wild-type (WT) and IL-10-deficient (Il-10−/−/ SvEv129) male/female mice (8 weeks of age) received 100 µL each of the human fecal suspension via oral gavage (one time, or three times on three consecutive days) using 20 Gauge gavage needle (Fine Science Tools). Human microbiota transplantation experiments were performed in 64 germ-free IL10−/− and 65 wild-type matching controls. A group of 4–6 Il-10−/− and 4–6 WT mice were tested per human donor colonization experiment (1x or 3x gavage). Colonized mice were housed in group-specific isolators reserved to mice colonized with the same human microbiota. Mice were killed 4 weeks after colonization.
For fecal microbiota transplantation, GF wild-type (WT) and IL-10-deficient (Il-10−/−/ SvEv129) male/female mice (8 weeks of age) received 100 µL each of the human fecal suspension via oral gavage (one time, or three times on three consecutive days) using 20 Gauge gavage needle (Fine Science Tools). Human microbiota transplantation experiments were performed in 64 germ-free IL10−/− and 65 wild-type matching controls. A group of 4–6 Il-10−/− and 4–6 WT mice were tested per human donor colonization experiment (1x or 3x gavage). Colonized mice were housed in group-specific isolators reserved to mice colonized with the same human microbiota. Mice were killed 4 weeks after colonization.
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