Human multiplex assay

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Human multiplex assay is a laboratory tool designed to simultaneously detect and quantify multiple analytes in a single biological sample. It allows for the simultaneous measurement of various proteins, cytokines, or other biomolecules, providing a comprehensive analysis of the sample.

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Lab products found in correlation

Luminex flexmap 3d, by Merck Group (2 mentions)

Spelling variants of this product

Multiplex assay (4 citations) Luminex multiplex human cytokine assay kit (3 citations) TaqMan miRNA Multiplex RT assays (human pool) (2 citations)

2 protocols using human multiplex assay

Quantitative Secretome Profiling of Caco-2 Cells

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Media was harvested separately form the top and bottom inlets and outlets, reflecting the apical and basal secretion of analytes by Caco-2 cells, and stored at −20 °C until further assessment. The concentrations of macrophage inflammatory protein-3 (CCL-20/MIP3A), IFN-Gamma-Inducible Protein 10 (IP-10/CXCL10), Interleukin-8 (IL-8/CXCL8), Interleukin-6 (IL-6), Interleukin-1-Beta (IL-1β) and Tumor Necrosis Factor-Alpha (TNF-α) were quantified using a human multiplex assay (Thermo Fischer, Waltham, MA, USA) on a Luminex FlexMap 3D (Merck Millipore, Burlington, MA, USA) according to manufacturer protocol.

Beaurivage C., Naumovska E., Chang Y.X., Elstak E.D., Nicolas A., Wouters H., van Moolenbroek G., Lanz H.L., Trietsch S.J., Joore J., Vulto P., Janssen R.A., Erdmann K.S., Stallen J, & Kurek D. (2019). Development of a Gut-on-a-Chip Model for High Throughput Disease Modeling and Drug Discovery. International Journal of Molecular Sciences, 20(22), 5661.

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Quantifying Inflammatory Cytokines in Effluents

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The concentration of Granulocyte–Macrophage Colony-Stimulating Factor (GM-CSF), Interleukin-12 unit p70 (IL-12p70), Interleukin-6 (IL-6), Interleukin-8 (IL-8/CXCL8), IFN-Gamma-Inducible Protein-10 (IP-10/CXCL10), Macrophage Inflammatory Protein-3 (CCL-20/MIP3A) and Tumor Necrosis Factor-Alpha (TNF-α) in collected effluents was determined by a human multiplex assay (Thermo Fischer) on a Luminex FlexMap 3D (Merck Millipore) according to manufacturer protocol.

Beaurivage C., Kanapeckaite A., Loomans C., Erdmann K.S., Stallen J, & Janssen R.A. (2020). Development of a human primary gut-on-a-chip to model inflammatory processes. Scientific Reports, 10, 21475.

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