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Fully automated biochemical analyzer

Manufactured by Hitachi
Sourced in Japan

The Fully Automated Biochemical Analyzer is a laboratory instrument designed to perform a wide range of biochemical tests and analyses. It automates the process of sample preparation, reagent addition, incubation, and detection, providing consistent and reliable results. The analyzer is capable of handling multiple samples simultaneously and can be programmed to conduct a variety of biochemical assays, making it a versatile tool for clinical laboratories, research facilities, and other settings where accurate and efficient biochemical analysis is required.

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3 protocols using fully automated biochemical analyzer

1

Liver Function Test Protocol

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Samples were processed for liver function test like ALT, AST, alkaline phosphatase (ALP), serum bilirubin, albumin, and globulin using specific kits (Swemed Diagnostics, Bangalore, India) according to manufacturer's instructions in fully Automated Biochemical Analyzer (Hitachi, Tokyo, Japan) (Table 1).
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2

Evaluating Kidney Injury Biomarkers

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Each mouse was sacrificed after the experiments were over. We collected blood samples from the right ventricle of the heart for biomechanical analyses performed later. We measured the levels of blood urea nitrogen (BUN), SCR, FBG, TG, and TC by a fully-automated biochemical analyzer (Hitachi). The urinary neutrophil gelatinase-associated lipocalin (u-NGAL, the marker for renal tubular injury) as well as serum AGEs were measured with an ELISA kit (CUSABIO; Wuhan, China). Meanwhile, to detect protein content from the urine collected at 24-h, this work conducted Coomassie brilliant blue protein assay (Jiancheng Bioengineering Institute, Nanjing, Jiangsu).
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3

Serum Lipid and Inflammatory Profiling

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Biochemical serum lipid profiles, including TC, TG, HDL-C, and LDL-C, were quantified using a fully automated biochemical analyzer (Hitachi). In addition, specific ELISA assay kits were used to quantify TNF-α and IL-6 levels in plasma.
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