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Integratedpyro q cpg software

Manufactured by Qiagen
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The IntegratedPyro-Q-CpG software is a tool used for the analysis of DNA methylation patterns. It provides quantitative and reproducible results by integrating data from the PyroMark Q24 system.

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Lab products found in correlation

Pyromark md system, by Qiagen (2 mentions)

2 protocols using integratedpyro q cpg software

1

Quantitative Methylation Analysis of HSD11B2 Gene

Check if the same lab product or an alternative is used in the 5 most similar protocols
A sodium bisulfite pyrosequencing approach was used to assess the
methylation status of HSD11B2. Characteristics of the assay
were assessed using dilution series of fully methylated referent DNA into
fully unmethylated referent DNA. The PyroMark MD system (QIAGEN Inc.,
Germantown, MD) was used for all pyrosequencing assays. Quantitative
assessment of the extent of DNA methylation at each of the 4
HSD11B2 CpG sites was performed by using the integrated
Pyro-Q-CpG software (QIAGEN Inc.) to analyze data using previously published
methods described further in Supplementary Material(online).(10 (link)) Genomic coordinates
are GRch37/hg19: 67464389, 67464395, 67464399, 67464412.Methylation
variables for the CpG sites were winsorized and natural log transformed and
analyzed as a continuous measure based on percent of methylation at each CpG
site.
Oni-Orisan O.O., Dansereau L.M., Marsit C.J., Smith L.M., Neal C.R., Della Grotta S.A., Padbury J.F, & Lester B.M. (2020). DNA methylation in Children with Prenatal Methamphetamine Exposure and Environmental Adversity. Pediatric research, 89(5), 1152-1156.
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2

Quantitative Methylation Analysis of HSD11B2 Gene

Check if the same lab product or an alternative is used in the 5 most similar protocols
A sodium bisulfite pyrosequencing approach was used to assess the
methylation status of HSD11B2. Characteristics of the assay
were assessed using dilution series of fully methylated referent DNA into
fully unmethylated referent DNA. The PyroMark MD system (QIAGEN Inc.,
Germantown, MD) was used for all pyrosequencing assays. Quantitative
assessment of the extent of DNA methylation at each of the 4
HSD11B2 CpG sites was performed by using the integrated
Pyro-Q-CpG software (QIAGEN Inc.) to analyze data using previously published
methods described further in Supplementary Material(online).(10 (link)) Genomic coordinates
are GRch37/hg19: 67464389, 67464395, 67464399, 67464412.Methylation
variables for the CpG sites were winsorized and natural log transformed and
analyzed as a continuous measure based on percent of methylation at each CpG
site.
Oni-Orisan O.O., Dansereau L.M., Marsit C.J., Smith L.M., Neal C.R., Della Grotta S.A., Padbury J.F, & Lester B.M. (2020). DNA methylation in Children with Prenatal Methamphetamine Exposure and Environmental Adversity. Pediatric research, 89(5), 1152-1156.
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