Log In Sign up
The largest database of trusted experimental protocols

Leptin ria

Manufactured by Linco Research
Visit Supplier

The Leptin RIA is a radioimmunoassay kit designed to quantitatively measure leptin levels in biological samples. Leptin is a hormone produced by adipose tissue that plays a role in regulating energy balance and metabolism.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Human Leptin RIA kit (16 citations) Leptin (10 citations) Multi-Species Leptin RIA kit (6 citations) Rat Leptin Ria Kit (2 citations) Multispecies leptin RIA (2 citations) LINCO Multispecies Leptin RIA (2 citations)

2 protocols using leptin ria

1

Measurement of Serum Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum omentin-1 was measured using an enzyme-linked immunosorbent assay (ELISA) (Biovendor, Candler, NC). The assay had an intra-assay coefficient of variation (CV) of 3.7%, and an inter-assay CV of 4.6%. The lower limit of detection was 2 ng/mL. Leptin was measured with a radioimmunoassay (Leptin RIA, Linco Research, St. Louis, MO; now Millipore, Billerica, MA) with an inter-assay CV of 6.2% and an intra-assay CV of 8.3%. Serum soluble TNF-α receptor II (sTNFRII) was measured with an ELISA (R & D Systems, Minneapolis, MN) with an intra-assay CV of 4.4%, inter-assay CV of 6.1% and a lower limit of detection of 7.8 pg/mL. Serum IL-6 was measured with an ELISA (R & D Systems, Minneapolis, MN) with an intra-assay CV of 7.4%, inter-assay CV of 7.8% and a lower limit of detection of 0.156 pg/mL. All samples were run in duplicates using standardized laboratory techniques. Stability of leptin and omentin-1 was confirmed with multiple freeze thaw cycles.
Hamnvik O.P., Thakkar B., Chamberland J., Aronis K., Schneider B, & Mantzoros C.S. (2014). Omentin-1 levels are reduced by pharmacologic doses of leptin, but remain unaffected by energy deprivation and display no day-night variation. International journal of obesity (2005), 39(2), 260-264.
+ Open protocol
+ Expand
2

Measurement of Serum Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum omentin-1 was measured using an enzyme-linked immunosorbent assay (ELISA) (Biovendor, Candler, NC). The assay had an intra-assay coefficient of variation (CV) of 3.7%, and an inter-assay CV of 4.6%. The lower limit of detection was 2 ng/mL. Leptin was measured with a radioimmunoassay (Leptin RIA, Linco Research, St. Louis, MO; now Millipore, Billerica, MA) with an inter-assay CV of 6.2% and an intra-assay CV of 8.3%. Serum soluble TNF-α receptor II (sTNFRII) was measured with an ELISA (R & D Systems, Minneapolis, MN) with an intra-assay CV of 4.4%, inter-assay CV of 6.1% and a lower limit of detection of 7.8 pg/mL. Serum IL-6 was measured with an ELISA (R & D Systems, Minneapolis, MN) with an intra-assay CV of 7.4%, inter-assay CV of 7.8% and a lower limit of detection of 0.156 pg/mL. All samples were run in duplicates using standardized laboratory techniques. Stability of leptin and omentin-1 was confirmed with multiple freeze thaw cycles.
Hamnvik O.P., Thakkar B., Chamberland J., Aronis K., Schneider B, & Mantzoros C.S. (2014). Omentin-1 levels are reduced by pharmacologic doses of leptin, but remain unaffected by energy deprivation and display no day-night variation. International journal of obesity (2005), 39(2), 260-264.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!