Anti gitr

Cell culture media was RPMI 1640 (Mediatech Inc.) supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine, and 50 mM 2-mercaptoethanol (Sigma-Aldrich). Fluorescent anti-CD4, anti-CD8α, anti-CD8β, anti-CD25, anti-CD44, anti-CD45, anti-CD45.1, anti-CD45.2, anti-CD62L, anti-CD103, anti-CCR4, anti-CCR6, anti-CCR7, anti-CCR-9, anti-TCRβ and anti-TCRγδ antibodies were purchased from Biolegend. Anti-CTLA-4, anti-PD-1, anti-GITR, anti-CD127, anti-IFNγ, anti-CXCR3 and anti-CXCR4 were purchased from BD Biosciences-Pharmingen. Anti-MHCII, anti-CD207, anti-Gr-1, anti-CD11b, and anti-Foxp3 staining kit were purchased from eBioscience. Murine recombinant IL-2, IL-6 and E-selection-FC were purchased from R&D Systems. BD Cytofix/Perm buffer was used for intracellular staining. Cells were run on a BD LSR II flow cytometer or a Beckman Coulter Gallios flow cytometer and analyzed by Flowjo (Flowjo LLC).