Cell culture medium, B27, fetal bovine serum, trypsin, and antibiotics were purchased from Gibco (Grand Island, NY, USA). Papain and DNase I were obtained from Sigma (St. Louis, Mo., USA). Lipofectamine 2000 was from Invitrogen (Grand Island, NY, USA). Antibodies against IGF-1, phosph-extracellular signal-regulated kinases 1 and 2 (phosph-ERK1/2), and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti-MBP was obtained from Abcam (Cambridge, England). Secondary antibodies were the products of Vector Lab (Burlingame, California, USA). Real-time polymerase chain reaction (RT-PCR) kit was purchased from Takara (Dalian, China). ERK inhibitor (i.e., PD98059) was from Promega (Madison, Wisconsin, USA). Other chemicals were the products of Sigma.
Real time polymerase chain reaction rt pcr kit
Manufactured by Takara Bio
Sourced in Japan, China
The Real-time polymerase chain reaction (RT-PCR) kit is a laboratory equipment product designed to amplify and detect specific DNA or RNA sequences in a sample. It enables the quantitative analysis of genetic material through real-time monitoring of the amplification process.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Cell culture medium, by Thermo Fisher Scientific (1 mentions)
Anti mbp, by Abcam (1 mentions)
Papain, by Merck Group (1 mentions)
Dnase 1, by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Agarose gel, by Takara Bio (1 mentions)
Fibronectin fn, by Merck Group (1 mentions)
Penicillin streptomycin p s, by ScienCell (1 mentions)
Trizol plus rna purification kit, by Tiangen Biotech (1 mentions)
Ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Tiangen Biotech (1 mentions)
Matrigel, by BD (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Acridine orange, by Beyotime (1 mentions)
One step primescript mirna cdna synthesis kit, by Takara Bio (1 mentions)
Sybr premix ex taq 2, by Takara Bio (1 mentions)
5 protocols using real time polymerase chain reaction rt pcr kit
1
Evaluating Intracellular Signaling Pathways
2
Anticancer Effects of BetA on Breast Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BetA was obtained from Tianjin Zhongxin Pharmaceutical Group Corporation Limited (China; purity >98%). Human breast carcinoma MDA-MB-231 and MDA-MB-468 cell lines were obtained from Foleibao Biological Technology Development Co. Ltd (China).13 (link) HMMECs, endothelial cell medium (ECM), foetal bovine serum (FBS), endothelial cell growth supplement (ECGS) and penicillin/streptomycin (P/S) for cell culture were purchased from ScienCell Research Laboratories (USA). L15, F15 and RPMI 1640 media for cell culture were purchased from Hyclone (USA).
Trypsin (0.25%) and ethylenediaminetetraacetic acid (EDTA; 0.02%) were purchased from Gibco (China), and dimethyl sulfoxide (DMSO) was purchased from Solarbio (China). Matrigel was obtained from BD Biosciences (USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) for MTT assay was purchased from Solarbio (China). RNA was extracted using TRIzol reagent and TRIzol plus RNA purification kit purchased from Tiangen (China). Agarose gel (1%) and real-time polymerase chain reaction (RT-PCR) kit were obtained from Takara (China). Fibronectin (FN) was obtained from Sigma (USA). Dulbecco’s Phosphate-Buffered Saline (DPBS) was obtained from Hyclone (USA).
Trypsin (0.25%) and ethylenediaminetetraacetic acid (EDTA; 0.02%) were purchased from Gibco (China), and dimethyl sulfoxide (DMSO) was purchased from Solarbio (China). Matrigel was obtained from BD Biosciences (USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) for MTT assay was purchased from Solarbio (China). RNA was extracted using TRIzol reagent and TRIzol plus RNA purification kit purchased from Tiangen (China). Agarose gel (1%) and real-time polymerase chain reaction (RT-PCR) kit were obtained from Takara (China). Fibronectin (FN) was obtained from Sigma (USA). Dulbecco’s Phosphate-Buffered Saline (DPBS) was obtained from Hyclone (USA).
3
Hela Cell Apoptosis Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cervical cancer Hela cells and Hela/DDP cells was obtained from the Laboratory of Obstetrics and Gynecology in Wuhan Union Hospital (China). Acridine orange and DDP freeze-dried powders were bought from Beyotime Biotechnology Co., Ltd. and Qilu Pharmaceutical Co, Ltd. (China), respectively. Dulbecco's modified Eagle medium (DMEM), TRIzol reagent and Lipofectamine2000 were obtained from Invitrogen (USA). Transcriptor first strand complementary deoxyribonucleic acid (cDNA) synthesis kit, real-time polymerase chain reaction (RT-PCR) kit, One Step Prime Script miRNA cDNA synthesis kit and SYBR Premix Ex Taq II were provided by TaKaRa (Japan). Real-time fluorescence quantitative amplification system was purchased from Applied Biosystems (USA). NanoDrop2000c protein nucleic acid detector was provided by Thermo (USA). Dimethyl sulfoxide (99.9%) was obtained from Sigma (USA). Fetal bovine serum (FBS) was bought from Procell Life Science and Technology Co, Ltd. (China). Fluorescein isothiocyanate cell apoptosis detection kit was provided by Nanjing Bio box Biotech. Co., Ltd. (China). The specific primers for miR-152, miR-152 mimic and internal reference U6 were all purchased from Qiagen (Germany).
4
Molecular Mechanisms in Cellular Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High glucose Dulbecco's modified Eagle's medium (DMEM; essential medium), heat‐inactivated fetus bovine serum (FBS), and streptomycin were purchased from Hyclone Laboratories Inc. Rabbit anti‐mouse cyclin D1 antibody was purchased from Abgent. Goat anti‐rabbit FAK antibody (FAK Polyclonal Antibody) was purchased from Proteintech Group. Anti‐calponin‐1 antibody, anti‐mTERF1 antibody, and electrochemiluminescence (ECL) detection reagents were purchased from Abcam (Cambridge, UK). β‐actin monoclonal antibody was purchased from Wuhan Biodiscover (Hubei, China). FAK siRNA and mTERF1 siRNA were synthesized by Guangzhou Ribobio Ltd. (Guangdong, China). TRIzol assay kit, Taka reverse transcription kit, and the real‐time polymerase chain reaction (RT‐PCR) kit were purchased from Takara (Japan). Human FAK and mTERF1 primers were purchased from Shanghai Sangon Ltd. (China). Human β‐actin primers were purchased from Guangzhou Ribobio Ltd. (China). Lipofectamine 2000 was purchased from Invitrogen.
5
Plasma Jet Assisted RNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The plasma jet device primarily consisted of an insulating shield made of pyrex tube through which the inert gas such as helium (99.99%) was injected to carry a reactive gas (5% oxygen) (Figure 1 ).
RNA extraction solution easy-BLUE (Total RNA Extraction Solution) was purchased from Intron Biotechnology (Gyeonggi-do, Republic of Korea), according to the manufacturer’s protocol (INTRON, cat17061). Complementary DNA (cDNA) synthesis was performed using Revert Aid TM First-Strand cDNA Synthesis Kit (ABI, cat4368814) according to the manufacturer’s instruction (Thermo Fisher Scientific, Waltham, MA, USA). Real-time polymerase chain reaction (RT-PCR) kit was supplied by Takara (Otsu, Japan). The human breast cancer cell lines (MCF-7) and human fibroblast (HF) primary cells were provided by the National Cell Bank Pasteur Institute (Tehran, Iran). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), and 4′,6-diamidino-2-phenylindole (DAPI) were provided by Thermo Fisher Scientific.
RNA extraction solution easy-BLUE (Total RNA Extraction Solution) was purchased from Intron Biotechnology (Gyeonggi-do, Republic of Korea), according to the manufacturer’s protocol (INTRON, cat17061). Complementary DNA (cDNA) synthesis was performed using Revert Aid TM First-Strand cDNA Synthesis Kit (ABI, cat4368814) according to the manufacturer’s instruction (Thermo Fisher Scientific, Waltham, MA, USA). Real-time polymerase chain reaction (RT-PCR) kit was supplied by Takara (Otsu, Japan). The human breast cancer cell lines (MCF-7) and human fibroblast (HF) primary cells were provided by the National Cell Bank Pasteur Institute (Tehran, Iran). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), and 4′,6-diamidino-2-phenylindole (DAPI) were provided by Thermo Fisher Scientific.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!